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| 538 files in 25 albums and 3 categories with 123 comments viewed 651966 times |
| Random files |

HIF-1alpha986 viewsHIF-1alpha staining of Zinc fixed, paraffin embedded xenograft tumor. Note nuclear staining of tumor cells.
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CD20749 viewsCD20 image.
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CD90 (Thy-1)1009 viewsCD90 (Thy-1) immunostaining on frozen tumor section. CD90 red. showing membrane/cytoplasmic staining pattern.
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Bim1008 viewsBim immunostaining.
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Mouse anti-human EGFR - example of PRECOMPLEXING1214 viewsThis is human tumor grown in mouse. I wanted to stain for EGFR w/ a mouse monoclonal antibody. Standard procedure would be staining mouse on mouse which would give background. Instead I precomplexed my mouse anti-human primary Ab w/ anti-mouse IgG-biotin secondary in a tube. Then (with some additional blocking) I added it to the mouse tissue and developed with Streptavidin-HRP and DAB+.
Observe the EGFR positivity in the epidermoid tumor & no staining in the mouse supportive stroma.
MaximinaMUA@yahoo.com
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Oct 3/4.1312 views4% PFA fixed cytospin stained with anti Oct-3/4 ab. No secondary ab is added, followed with Avidin labelled FITC. and counterstained with PI.
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Luxol Fast Blue for Myelin4098 viewsLuxol fast blue staining of rat brain. Note myelinated axons stained blue.
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tra2ß660 viewstra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB.
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| Last additions |

Biomoles GFAP IHC Staining 295 viewsBiomoles ready-to-use GFAP monoclonal antibody displayed rare 100% specificity in brain tissue astrocytes only. All other cell types and background tissue exhibited entire negativity. Apr 24, 2009
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GFAP IHC 100% Specificity 425 viewsBiomoles ready-to-use GFAP monoclonal antibody displayed rarely seen 100% specificity in brain tissue on astrocytesApr 24, 2009
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CASR IHC validation 227 viewsCASR IHC cytomembrane stain, antibody validated by Biomoles, Inc. Mar 26, 2009
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Ki-67 IHC validation 357 viewsKi-67 IHC tonsil lymphocytes stain, antibody validated by Biomoles, Inc. Mar 26, 2009
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GFAP IHC validation 281 viewsGFAP IHC stain, antibody provided and validated by Biomoles, Inc. Mar 26, 2009
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Ki-67 IHC validation 446 viewsKi-67 IHC nuclear stain, antibody provided and validated by Biomoles, Inc. Mar 26, 2009
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water lily 210 viewsmiraculous image of the moon from God
Photo provided by Shici. Copyright reserved by the author Mar 25, 2009
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water lily 120 viewsmiraculous image of the moon from God
Photo provided by Shici. Copyright reserved by the author Mar 25, 2009
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