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Andrey Khramtsov's Gallery

It is possible to use standard microtome sectioning
techniques for cutting the tissue microarray block |

Formalin fixed, paraffin embedded human tissue (invasive
breast carcinoma) stained with HER-2/neu (DAKO) |

Preparation of the array block |

Breast carcinoma stained with Estrogen Receptor anibody,
code M7047 |

Array block with 162 punches, which is constructed using
ATA-27 automated arrayer |
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herpes's Gallery

Staining of breats cancer cells using anti-WIP1 ab. dilution
1:2000 |

Immunofluorescence staining with PDGFR alpha (phospho Y754)
antibody |

Breast carcinoma biopsy stained with anti-WIP-1 antibody |

Benign prostate biopsy stained with anti-PDGFR alpha
(phospho Y754) antibody |

Induction of PDGFR-alpha phosphorylation |

Benign prostate biopsy stained with anti-PDGFR alpha
(phospho Y754) antibody |
MaximinaNYC's Gallery
Contact me
at MaximinaMUA@yahoo.com or mail me on this site for more
information about protocols etc.

Anti-alpha Smooth Muscle Actin (SMA) on mouse teratoma
I used clone 1A4 EPOS labeled from DAKO to stain mouse
teratoma grown in NOD-SCID mice. AEC+ is the chromagen. Note
the intense and specific staining of the smooth muscle cells
surrounding an area of differentiated cells within the tumor |

Anti-alpha Smooth Muscle Actin (SMA) on mouse teratoma
I used clone 1A4 EPOS labeled from DAKO to stain mouse
teratoma grown in NOD-SCID mice. AEC+ is the chromagen. Note
the intense and specific staining of the smooth muscle cells
surrounding distinct regions of differentiated ductal
epithelial cells within the tumor |

B220 B cell marker in frozen mouse spleen
Anti-B cell B220 immunohistochemistry of acetone fixed mouse
spleen using a rat monoclonal. Note the follicular
localization of B220 positive B cells. |

Cleaved caspase 3 on FFPE Human Tonsil
Rabbit anti-human cleaved caspase-3 IHC on human tonsil.
EDTA retrieval was necessary to achieve these results. Note
cytoplasmic and punctate localization of immunoreactivity |

Gr-1 granulocyte marker in frozen mouse spleen
Anti-Gr-1 immunohistochemistry of acetone fixed mouse spleen
using a rat monoclonal. Note the peri-follicular
localization of Gr-1 |

Ki67 in mouse FFPE
This is a stain of proliferating cells (not in G0)
immunostained with anti-mouse Ki67 antibody, clone Tec-3, in
FFPE mouse spleen. Note the crisp nuclear immunoreactivity
of Ki67+ cells |

Mouse anti-human EGFR - example of PRECOMPLEXING
This is human tumor grown in mouse. I wanted to stain for
EGFR w/ a mouse monoclonal antibody. Standard procedure
would be staining mouse on mouse which would give
background. Instead I precomplexed my mouse anti-human
primary Ab w/ anti-mouse IgG-biotin secondary in a tube.
Then (with some additional blocking) I added it to the mouse
tissue and developed with Streptavidin-HRP and DAB+. Observe
the EGFR positivity in the epidermoid tumor & no staining in
the mouse supportive stroma. |

Pan-endothelial cell antigen in FFPE Mouse Kidney
Vessel IHC of FFPE mouse kidneys using a rat anti-mouse pan
endothelial cell antigen antibody. Developed with AEC+. Note
the staining of peri-tubular endothelium but an absence of
glomerular immunoreactivity. This patchy staining represents
a major problem with using this clone broadly |

TSP on FFPE Mouse Tissue
Thrombospondin IHC of FFPE mouse bone marrow using a mouse
anti-human TSP antibody which cross reacts to human.
Developed with AEC+. Note the staining of megakaryocytes and
platelet particles. |

Martius Scarlet Blue - Normal Mouse Bone Marrow
This is normally a stain for fibrin. In this case I stained
normal FFPE mouse bone marrow for MSB. Note the red staining
muscle, blue staining bone, black bone marrow and virtual
absence of red fibrin in the healthy bone marrow proper. |
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molonk's Gallery

tra2ß in cervical carcinoma, developed with a Vectastain Kit
and DAB |

tra2ß in cervical carcinoma, developed with a Vectastain Kit
and DAB |

tra2ß in cervical carcinoma, developed with a Vectastain Kit
and DAB |

tra2ß in cervical carcinoma, developed with a Vectastain Kit
and DAB |

tra2ß in cervical carcinoma, developed with a Vectastain Kit
and DAB |
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n_bjorkhammer's Gallery
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CD45 in tonsil |

PSA |

SMA,
Increased background
in glandular tissue
reason behind this? |