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Andrey Khramtsov's Gallery

It is possible to use standard microtome sectioning techniques for cutting the tissue microarray block	Formalin fixed, paraffin embedded human tissue (invasive breast carcinoma) stained with HER-2/neu (DAKO)	Preparation of the array block
Breast carcinoma stained with Estrogen Receptor anibody, code M7047	Array block with 162 punches, which is constructed using ATA-27 automated arrayer

herpes's Gallery

Staining of breats cancer cells using anti-WIP1 ab. dilution 1:2000	Immunofluorescence staining with PDGFR alpha (phospho Y754) antibody	Breast carcinoma biopsy stained with anti-WIP-1 antibody
Benign prostate biopsy stained with anti-PDGFR alpha (phospho Y754) antibody	Induction of PDGFR-alpha phosphorylation	Benign prostate biopsy stained with anti-PDGFR alpha (phospho Y754) antibody

MaximinaNYC's Gallery

Contact me at MaximinaMUA@yahoo.com or mail me on this site for more information about protocols etc.

Anti-alpha Smooth Muscle Actin (SMA) on mouse teratoma I used clone 1A4 EPOS labeled from DAKO to stain mouse teratoma grown in NOD-SCID mice. AEC+ is the chromagen. Note the intense and specific staining of the smooth muscle cells surrounding an area of differentiated cells within the tumor	Anti-alpha Smooth Muscle Actin (SMA) on mouse teratoma I used clone 1A4 EPOS labeled from DAKO to stain mouse teratoma grown in NOD-SCID mice. AEC+ is the chromagen. Note the intense and specific staining of the smooth muscle cells surrounding distinct regions of differentiated ductal epithelial cells within the tumor	B220 B cell marker in frozen mouse spleen Anti-B cell B220 immunohistochemistry of acetone fixed mouse spleen using a rat monoclonal. Note the follicular localization of B220 positive B cells.
Cleaved caspase 3 on FFPE Human Tonsil Rabbit anti-human cleaved caspase-3 IHC on human tonsil. EDTA retrieval was necessary to achieve these results. Note cytoplasmic and punctate localization of immunoreactivity	Gr-1 granulocyte marker in frozen mouse spleen Anti-Gr-1 immunohistochemistry of acetone fixed mouse spleen using a rat monoclonal. Note the peri-follicular localization of Gr-1	Ki67 in mouse FFPE This is a stain of proliferating cells (not in G0) immunostained with anti-mouse Ki67 antibody, clone Tec-3, in FFPE mouse spleen. Note the crisp nuclear immunoreactivity of Ki67+ cells
Mouse anti-human EGFR - example of PRECOMPLEXING This is human tumor grown in mouse. I wanted to stain for EGFR w/ a mouse monoclonal antibody. Standard procedure would be staining mouse on mouse which would give background. Instead I precomplexed my mouse anti-human primary Ab w/ anti-mouse IgG-biotin secondary in a tube. Then (with some additional blocking) I added it to the mouse tissue and developed with Streptavidin-HRP and DAB+. Observe the EGFR positivity in the epidermoid tumor & no staining in the mouse supportive stroma.	Pan-endothelial cell antigen in FFPE Mouse Kidney Vessel IHC of FFPE mouse kidneys using a rat anti-mouse pan endothelial cell antigen antibody. Developed with AEC+. Note the staining of peri-tubular endothelium but an absence of glomerular immunoreactivity. This patchy staining represents a major problem with using this clone broadly	TSP on FFPE Mouse Tissue Thrombospondin IHC of FFPE mouse bone marrow using a mouse anti-human TSP antibody which cross reacts to human. Developed with AEC+. Note the staining of megakaryocytes and platelet particles.
Martius Scarlet Blue - Normal Mouse Bone Marrow This is normally a stain for fibrin. In this case I stained normal FFPE mouse bone marrow for MSB. Note the red staining muscle, blue staining bone, black bone marrow and virtual absence of red fibrin in the healthy bone marrow proper.

molonk's Gallery

tra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB	tra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB	tra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB
tra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB	tra2ß in cervical carcinoma, developed with a Vectastain Kit and DAB

n_bjorkhammer's Gallery

CD45 in tonsil

PSA

SMA, Increased background
in glandular tissue
reason behind this?