Introduction to Immunohistochemistry




Immunohistochemistry is the localization of antigens or proteins in tissue sections by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, or colloidal gold.



Tissue Preparation (Fixation, Sectioning, Whole Mount Preparation)

Antigen Retrieval

IHC Methods (Blocking, Controls, Direct Method, Indirect Method, PAP Method, ABC Method, LSAB Method, Polymeric Method, CSA Method, Sensitivity Chart)

Multiple Labeling

Immuno-Electron Microscopy