neuromethods

techniques for neuroscientists

welcome to neuromethods

Neurohistology

General Histology, Special Stains, Histochemistry, Immunochemistry, In Situ Hybridization, Axonal Tracing of Neuronal Connections, Light Microscopy, Confocal Laser Microscopy, Electron Microscopy.

Neurophysiology

Single Unit Recording, Intracellular Recording, Extracellular Single Neuron Recording, Massed Electrical Recording, Electroencephalogram (EEG), Patch Clamping, Brain Slice, Microdialysis, Measurement of Ion Concentration.

Neuron Culture

Cortical Neuron Culture, Glia Cell Culture, Organotypic Cultures, Flow Cytometry, Transfection in Cell Cultures, Drug Application.

Molecular Neurobiology

DNA and RNA, Transcription, Translation, Gene Cloning, Nucleic Acid Sequencing, PCR, Reverse Transcription-PCR (RT-PCR)

Protein Chemistry

 

Protein Production, Purification, Expression, GAS Chromatography, Gel Electronphoresis, High Performance Liquid Chromatography, Immunoblotting, Western Blotting, ELISA.

 

Genetic Methods

 

Genetic Mutations, Gene Knockout, Gene Knockin, Gene Knockdown, Gene Expression, Creation of Transgenic Mice.

 

Brain Imaging

 

CAT Scan (Computerized Atom Tomography), CT Scan (Computerized Tomography), MRI (Magnetic Resonance Imaging), PET Scan (Positron Emission Tomography), 2-DG (2-Deoxyglucose), fMRI (functional MRI), nMRI (nuclear MRI), X-Ray Crystallography, Computer Modeling, Digital Signal Processing.

 

Neurosurgery

 

Stereotaxic Surgery, Lesioning Techniques, Ablation, Brain Microinjections, Pharmacological/Biochemical Lesions, Neural Grafting, Drug Delivery.

Image Analysis

Image Analysis, Stereological Analysis.

Neural Networks

Artificial Neural Networks.

Cognitive & Behavioral

Motor Rotational Test, Learning and Memory Test, Language, Problem Solving.

Animal Models

Parkinson's Disease, Alzheimer Disease, Brain Ischemia, Brain Stroke, Peripheral Nerve Injury.