neuromethods

genetic methods

 

 

Transgenic/Gene Knockin

• What is a Transgenic Mouse? (UC-Irvine)

• Transgenic Animals - Methods and Techniques

• Transgenic Animals - Historical Background and Methods of Creation of Transgenic Animals

• Transgenic Animals - for basic research and biotechnology (California State University Northridge)

• Transgenic Methods: The Illuminating Power & Controversy by Paul T. Toran

• Lineage Analysis Using Retroviral Vectors (Cepko/Tabin Lab Protocols, Harvard Medical School and Howard Hughes Medical Institute)

Gene Knockout

• Knockout Mice - What is a knockout mouse?

• Transgenes and Knockouts - How scientists are exploring the genetic universe

• Elimination of Gene - Gene Knock-Out

• Gene Knockout Techniques (Biotechnology Laboratory, University of British Columbia)

• Homologous Recombination Method
  (and Knockout Mouse)
• A novel method for constructing gene-targeting vectors
• Gene Knockout with Conventional Mutagens by Leon Avery
• Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses
• Gene Targeting Outline (University of Michigan Transgenic Animal Model Core)

Gene Knockdown

• RNAi Frequently Asked Questions - In RNAi experiments, researchers usually refer to “knockdown” of gene expression rather than the more classical “knockout”

• Local gene knockdown in the brain using viral-mediated RNA interference (Nature Medicine Technical Report)

• Neurochemical and behavioral consequences of widespread gene knockdown in the adult mouse brain by using nonviral RNA interference

• A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA

• Knockdown of chimeric glucocerebrosidase by green fluorescent protein-directed small interfering RNA

• RNAi-induced gene silencing by local electroporation in targeting brain region

• Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown

• Gene knockdown with intrathecal siRNA of NMDA receptor NR2B subunit reduces formalin-induced nociception in the rat

• Selective knockdown of gene expression of N-methyl-D-aspartate receptor one ameliorates parkinsonian motor symptom in 6-hydroxydopamine-lesioned rats

• Lentiviral vector-mediated delivery of short hairpin RNA results in persistent knockdown of gene expression in mouse brain

• In vivo knockdown of gene expression in brain cancer with intravenous RNAi in adult rats

• Antisense in vivo knockdown of synaptotagmin I and synapsin I by HVJ-liposome mediated gene transfer modulates ischemic injury of hippocampus in opposing ways

 

 

 

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