neuromethods

molecular neurobiology

 

 

Molecular Biology Manuals

• Beginning Molecular Biology Laboratory Manual (Department of Biological Sciences, UMBC)

• Molecular Biology Methods - Protocols for Recombinant DNA Isolation, Cloning, and Sequencing by Bruce A. Roe, et al (Department of Chemistry and Biochemistry, The University of Oklahomas)

• Jim Brown's Molecular Biology Materials and Methods

• Cloning from Beginning to End (Finkbeiner Lab, UCSF)

DNA Methods

• Mini-Plasmid Preparation Protocol of D. Grant McFadden

• CsCl Prep of Plasmid DNA - a Standard Large Scale Prep for Plasmid DNA (Mike A. Dyer, Harvard Medical School)

• DNA Plasmid Prep (Mike A. Dyer, Harvard Medical School)

• Quick DNA Plasmid Prep - Boiling 10 minute protocol (Mike A. Dyer, Harvard Medical School)

• Quick DNA Plasmid Prep - NaOH/SDS protocol (Mike A. Dyer, Harvard Medical School)

• Lambda DNA Preparation (Mike A. Dyer, Harvard Medical School)

• DNA Fragment Purification from Agarose or Acrylamide (Mike A. Dyer, Harvard Medical School)

• Single Strand DNA Prep. for Sequencing (Mike A. Dyer, Harvard Medical School)

RNA Methods

• Total RNA Isolation Protocol (BD Biosciences)

• RNase Protection Assay (BD Bioseciences)

• RNA Preparation from Cultured Cells or Tissue Samples (Mike A. Dyer, Harvard Medical School)

• RNase Protection Assay (Mike A. Dyer, Harvard Medical School)

PCR/RT-PCR

• PCR Genotyping from Tail DNA (Mike A. Dyer, Harvard Medical School)

• RT-PCR Analysis - Semi-quantitative (Mike A. Dyer, Harvard Medical School)

Southern Blotting

• Southwestern Blot for DNA binding proteins (Mike A. Dyer, Harvard Medical School)

Western Blotting

• Western Blots Protocol (Mike A. Dyer, Harvard Medical School)

 

 

 

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