neuromethods

neurohistology

 

 

General Histology

• Histological processing (including fixation), embedding, sectioning, H&E staining (The Internet Pathology Laboratory)

• Brain Tissue Fixation and Storage General Protocols (Primate Brain Bank)

• Parraffin Sectioning Protocol (Mike A. Dyer, Harvard Medical School)

• Cryosectioning Protocol (Mike A. Dyer, Harvard Medical School)

Special Stains

• Bielschowsky Method for Senile Plaques and Tangles (University of Nottingham)

• Bielschowsky Technique - Senile Plaques (The Internet Pathology Laboratory)

• Bodian's Method - Nerve Fibers (The Internet Pathology Laboratory)

• Congo Red - Putchler's Modification - Amyloid (The Internet Pathology Laboratory)

• Glial Fibers - Holzer's Stain (The Internet Pathology Laboratory)

• Luxol Fast Blue - Modified Kluver's - Myelin Sheath (The Internet Pathology Laboratory)

• Nissl Substance - Cresyl Echt Violet Stain (The Internet Pathology Laboratory)

• Thiolfavin S Stain for Amyloid in Tissues (The Internet Pathology Laboratory)

• Modified Thiolfavin S Stain for Senile Plaques (The Internet Pathology Laboratory)

• Staining Methods for cell death (Z. Xia, UCSF)

• Protocol for Fluoro-Jade Histofluorescent Labeling of Degenerating Neurons (Developed and Maintained by Tom Hallam)

• Co-staining with Thioflavin S & Carboxyfluorescein (Dr. KA Crutcher, University of Cincinnati)

Tract-Tracing

• Dyes and tracers: examples for popularly used neural dyes and tract-tracing compounds

• Tract-tracing in the nervous system of vertebrates using horseradish peroxidase and its conjugates: tracers, chromogens and stabilization for light and electron microscopy

• Retrograde tracing with Fluoro-Gold: different methods of tracer detection at the ultrastructural level and neurodegenerative changes of back-filled neurons in long-term studies

• Immunolabeling of retrogradely transported Fluoro-Gold: sensitivity and application to ultrastructural analysis of transmitter-specific mesolimbic circuitry

• Rapid and reproducible methods using fluorogold for labelling a subpopulation of cervical motoneurons: application in the wobbler mouse

• A Novel Method for Simultaneous Anterograde and Retrograde Labeling of Spinal Cord Motor Tracts in the Same Animal

Enzyme Chemistry

• Beta-Gal staining in primary neurons (Finkbeiner Lab, UCSF)

• Detection of beta-Galactosidase and Alkaline Phosphatase Activities in Tissue (Cepko/Tabin Lab Protocols, Harvard Medical School and Howard Hughes Medical Institute)

• Histochemical Staining: AP and Beta-galactosidase (Mike A. Dyer, Harvard Medical School)

Immunohistochemistry

• Immunocytochemistry In Free-Floating Sections

• Dissociated Retina Immunohistochemistry (Mike A. Dyer, Harvard Medical School)

• Tyramide Amplification and Synthesis for Immunohistochemistry (Mike A. Dyer, Harvard Medical School)

In Situ Hybridization

• In situ Hybridization (Sean M. Montgomery, Rutgers University)

• Protocols: mRNA In Situ Hybridization with Nick-Translated Probes (Spector Lab)

• RNA Whole Mount In Situ Hybridization (Cepko/Tabin Lab Protocols, Harvard Medical School and Howard Hughes Medical Institute)

Fluorescence Microscopy

• Fluorescene Microscopy

• Fluorescence Mounting Medium (Spector Lab)

• Protocols: Immunofluroescence Technique (Spector Lab)

Electron Microscopy

• Protocols: Post-Embedding Immunogold Method (Spector Lab)

 

 

 

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