neuromethods

neuron culture

 

 

General Methods

• Bart's Cookbook and Lab Protocols - Tissue Culture

Buffers and Recipes

• Conditioned Medium for Retinal Explant Culture (Mike A. Dyer, Harvard Medical School)

• Nuclear Extract Preparation (Mike A. Dyer, Harvard Medical School)

Neuron Cultures

• Stable Lines (Mike A. Dyer, Harvard Medical School)

• Cell Culture Protocol - A good guide for preparing Dorsal Horn neuron monocultures, Dorsal Horn and Dorsal Root Ganglion neuron Co-cultures and microisland cultures (MacDermott Lab, Columbia University)

• Dissociated Cultures of Cerebellar Neurons (Hank Dudek, UCSF)

• Successfully culturing Schwann cells from adult peripheral nerve (A.D. Ansselin, et al., University of Sydney)

• Co-culturing neurons and microglia using tissue culture inserts (Tsirka Lab, Stony Brook)

• Hippocampal Neuron Cultures and Mixed Cortical Cultures (Tsirka Lab, Stony Brook)

• Culture of Dissociated Sympathetic Neurons (Crutcher Lab, University of Cincinnati Medical Center)

• Culture of Dissociated Cortical Neurons Culture (Crutcher Lab, University of Cincinnati Medical Center)

• Organotypic Hippocampal Slice Cultures (Panasonic MED64 Systems)

Transfection Techniques

• COS Cell Transfection (Mike A. Dyer, Harvard Medical School)

• Phoenix Transfection/Viral Production (A) (Mike A. Dyer, Harvard Medical School

 

 

 

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