neuromethods

protein chemistry

 

 

General Methods Overview

• Bart's Cookbook and Lab Protocols - Protein Biochemistry

Buffers and Recipes

• Column Buffers: CREB (Finkbeiner Lab, UCSF)

• Lysis Buffers (Finkbeiner Lab, UCSF)

• Immunoprecipitation Buffers and Protocols (Finkbeiner Lab, UCSF)

• Cell Lysis/Western/IP Methods (Finkbeiner Lab, UCSF)

• Gel Drying Method (Finkbeiner Lab, UCSF)

• SDS-PAGE Method and Recipe (Finkbeiner Lab, UCSF)

Protein Production

• Baculovirus Production for Recombinant Proteins (Mike A. Dyer, Harvard Medical School)

• Electroelution of Proteins From SDS-PAGE Gels (Mike A. Dyer, Harvard Medical School)

Protein Purification

• Preparation of Affinity Column (Finkbeiner Lab, UCSF)

• Immunoaffinity Purification of Antibodies (Finkbeiner Lab, UCSF)

• Antibody Purification Protocol (Mike A. Dyer, Harvard Medical School)

• Guanidine Hydrochloride Purification of Proteins From SDS PAGE (Mike A. Dyer, Harvard Medical School)

• Expression & Purification of His Tagged Proteins in E. coli (Mike A. Dyer, Harvard Medical School)

Protein Expression

• Ponceau Staining of Nitrocellulose (Mike A. Dyer, Harvard Medical School)

• Protein Gel Preparation and Staining (Mike A. Dyer, Harvard Medical School)

• CuCl2 Staining of SDS-PAGE Gels (Mike A. Dyer, Harvard Medical School)

• Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Dr. KA Crutcher, University of Cincinnati)

Western Blotting

• Online Western Blot Protocol Resource with protocols (from protein extraction to Bradford assays), troubleshooting, buffer recipes and tips to solve immunoblot problems

• Western Blotting with Alkaline Phosphatase Conjugates (BD Biosciences)

• Western Blotting with Biotinylated Antibodies (BD Biosciences)

• Western Blotting with Horseradish Peroxidase Conjugates (BD Biosciences)

• Western Blotting with Monoclonal Antibodies (BD Biosciences)

• Western Blotting with Rabbit Polyclonal Antibodies (BD Biosciences)

• Preparation of Brain Membrane Fractions for Western Blot Analysis (BD Biosciences)

• Western Blot/Anti-P-CREB (Finkbeiner Lab, UCSF)

• Protocol for anti-HA antibody Western Blotting (Marc Diamond’s method)

• Western blotting protocol for 1C2, 3B5H10, and 4C8 Antibodies

 

 

 

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