NovaUltraTM

Special Stain Kits

 

Histology special stains use reagents and solutions made from histological dyes to identify and demonstrate specific structures and cellular components that aid pathologists in the evaluation of certain diseases. IHC World offers the most commonly used special stain kits for your research needs. All of our special stain kits contains solutions that are pre-diluted, ready to use form. A step by step protocol is provided for each kit for the convenience of users. IHC World also offers the biological dyes for customers wishing to prepare their own staining solutions.

 

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The place to purchase our products online

 


 

How to Order

NovaUltraTM H&E Stain Kit

Special formulated hematoxylin and eosin solution for quality and fast H&E staining with no need to perform bluing nor clearing/differentiation steps. Nuclei are stained blue crispy clear color with pink cytoplasmic and background color.

 


Catalog# IW-3100

Unit: Kit

Price: $195

 

The kit components:

  • Mayer's Hematoxylin Solution - 250 ml

  • Eosin Solution - 250 ml

NovaUltraTM Alcian Blue Stain Kit

This kit is designed to stain formalin fixed, paraffin embedded tissue sections, and may be used for frozen sections as well. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink.

 


Catalog# IW-3000

Unit: Kit

Price: $195

 

The kit components:

  • Alcian Blue Solution - 250 ml

  • Nuclear Fast Red Solution - 250 ml

NovaUltraTM Alcian Blue - PAS Stain Kit

NovaUltra Alcian Blue – PAS Stain KitThis kit utilizes the properties of both the PAS and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. The rationale of the technique is that by first staining all the acidic mucins with Alcian Blue, those acidic mucins which are also PAS positive will be chemically blocked and will not react further during the technique. Those neutral mucins which are solely PAS positive will subsequently be demonstrated in a contrasting manner. The combined result demonstrates both acidic and neutral mucins as well as mixtures of acidic and neutral mucins.


Catalog# IW-3019

Unit: Kit

Price: $295

 

The kit components:

  • Alcian Blue Solution - 250 ml

  • Schiff Reagent - 250 ml

  • Periodic Acid Solution - 250 ml

  • Mayer's Hematoxylin Solution - 250 ml

NovaUltraTM Alizarin Red Stain Kit

Alizarin Red S, an anthraquinone derivative, may be used to identify calcium in tissue sections. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is birefringent.

 


Catalog# IW-3001

Unit: Kit

Price: $95

 

The kit components:

  • Alizarin Red Solution - 250 ml

NovaUltraTM Congo Red Stain Kit

This kit is a modified Highman's Congo Red stain. It is used for the detection of amyloid on formalin-fixed, paraffin-embedded tissue sections with amyloidosis, and may be used for frozen sections as well. The amyloid deposits will be stained red and the nuclei will be stained blue. The thickness of sections is usually 5 um. But in case of inadequate amyloid deposits, 10um thick sections will be more satisfactory.

 


Catalog# IW-3002

Unit: Kit

Price: $195

 

The kit components:

  • Congo Red Solution - 250 ml

  • Alkaline Alcohol Solution - 250 ml

  • Mayer’s Hematoxylin Solution - 250 ml

NovaUltraTM Fontana Masson Stain Kit

Fontana-Masson stains argentaffin granules and melanin. Melanin is a nonlipid, nonhematogenous pigment. It is a brown-black pigment present normally in the hair, skin, retina, iris, and certain parts of CNS. Argentaffin granules are found in carcinoid tumors. This kit is designed to stain formalin fixed, paraffin embedded tissue sections, and may be use for frozen sections as well. The argentaffin cell granules or melanin will be stained black. The nuclei will be stained pink-red. The cytoplasm will be stained pale pink.

 


Catalog# IW-3003

Unit: Kit

Price: $295

 

The kit components:

  • FM Silver Nitrate Solution - 100 ml

  • Gold Chloride Solution - 100 ml

  • Sodium Thoisulfate Solution - 100 ml

  • Nuclear Fast Red Solution - 100 ml

NovaUltraTM Golgi-Cox Stain Kit

The Golgi-Cox Kit is designed for the staining of neuronal dendrites and dendritic spines on fresh or paraformaldehyde fixed brain tissues. The Kit has been extensively tested on both fresh and paraformaldehyde fixed brain tissues in rats and mice and should work on other species as well. The impregnation will take 10-14 days depending on the age and size of the tissues. The Kit only contains two ready-to-use reagents and the protocol is easy to follow and the staining quality is excellent.


Catalog# IW-3023

Unit: Kit

Price: $295

 

The kit components:

  • Golgi-Cox Solution - 250 ml

  • Post-Impregnation Solution - 250 ml

NovaUltraTM Hema-Diff Stain Kit

NovaUltra Hema-Diff™ Stain Kit

Three step procedure for differential staining of blood smears. Used to evaluate blood parasites, microbial organisms and bone marrow specimens, Hema-Diff™ is used extensively in FNA Cytology and Veterinary Medicine. The entire procedure takes 30 seconds as air dried specimens are fixed in Solution 1 then dipped in Solutions 2 and 3. Staining intensity is easily manipulated. Three step procedure for hematological staining, FNA staining, and used as a special stain for helicobacter pylori.

 


Catalog# IW-3017

Unit: Kit

Price: $195

 

The kit components:

  • Hema-Diff Solution 1-Fixative - 250 ml

  • Hema-Diff Solution 2-Xanthene - 250 ml 

  • Hema-Diff Solution 3-Thaizine - 250 ml

NovaUltraTM Luxol Fast Blue Stain Kit

This kit is designed for staining myelin/myelinated axons on formalin-fixed, paraffin-embedded brain and spinal cord tissue sections, as well as frozen sections. The myelin, including phospholipids, will be stained blue to green, and the neurons will be stained violet. This stain is commonly used for identifying the basic neuronal structure in brain or spinal cord tissue. 

 


Catalog# IW-3005

Unit: Kit

Price: $195

 

The kit components:

  • Luxol Fast Blue Solution - 250 ml

  • Cresyl Violet Solution - 250 ml

  • Lithium Carbonate Solution - 250 ml

NovaUltraTM Masson Trichrome Stain Kit

This kit is used for the detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The collagen fibers will be stained blue and the nuclei will be stained black and the background is stained red.


Catalog# IW-3006

Unit: Kit

Price: $295

 

The kit components:

  • Aniline Blue Solution - 250 ml

  • Biebrich Scarlet-Acid Fuchsin Solution - 250 ml

  • Weigert's Iron Hematoxylin - 250 ml

  • Phosphomolybdic-Phosphotungstic Acid Solution - 250 ml

  • Acetic Acid Solution - 250 ml

NovaUltraTM Nissl Stain Kit

This kit is used for the detection of Nissl body in the cytoplasm of neurons on formalin-fixed, paraffin embedded tissue sections, as well as frozen sections. The Nissl body will be stained purple-blue. This stain is commonly used for identifying the basic neuronal structure in brain or spinal cord tissue. 

 


Catalog# IW-3007

Unit: Kit

Price: $95

 

The kit components:

  • Cresyl Violet Solution - 250 ml

NovaUltraTM Oil Red O Stain Kit

This kit is for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections.

 


Catalog# IW-3008

Unit: Kit

Price: $195

 

The kit components:

  • Oil Red O Solution - 100 ml

  • Pre-Stain Solution - 100 ml

  • Differentiation - Solution - 100 ml

  • Mayer's Hematoxylin Solution - 100 ml

NovaUltraTM Orcein Elastin Stain Kit

NovaUltra Orcein Elastin Stain Kit

This kit is used for identifying elastic fibers on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The elastic fibers will be stained dark brown and the nuclei will be stained light blue. The Acid Orcein Solution has also been show to stain the rough endoplasmic reticulum of hepatitis B infected liver cells (ground glass cells).

 


Catalog# IW-3018

Unit: Kit

Price: $195

 

The kit components:

  • Acid Orcein Solution - 250 ml

  • Mayer's Hematoxylin Solution - 250 ml

NovaUltraTM PAS Stain Kit

This kit is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The glycogen, mucin, and fungi will be stained purple and the nuclei will be stained blue.

 


Catalog# IW-3009

Unit: Kit

Price: $195

 

The kit components:

  • Schiff Reagent - 250 ml

  • Periodic Acid Solution - 250 ml

  • Mayer’s Hematoxylin Solution - 250 ml

NovaUltraTM Prussian Blue Stain Kit

Prussian blue reaction involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (+3) present in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called Prussian blue. This is one of the most sensitive histochemical tests and will demonstrate even single granules of iron in blood cells.

 


Catalog# IW-3010

Unit: Kit

Price: $195

 

The kit components:

  • Hydrochloric Acid Solution - 125 ml

  • Potassium Ferrocyanide Solution - 125 ml

  • Nuclear Fast Red Solution - 250 ml

NovaUltraTM Safranin O Stain Kit

This kit is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The cartilage and mucin will be stained orange to red, and the nuclei will be stained black. The background is stained green.

 


Catalog# IW-3011

Unit: Kit

Price: $295

 

The kit components:

  • Safranin O Solution - 250 ml

  • Weigert's Iron Hematoxylin Solution - 250 ml

  • Fast Green Solution - 250 ml

  • Acetic Acid Solution - 250 ml

NovaUltraTM Picro-Sirius Red Stain Kit

It's one of the best understood techniques of collagen histochemistry.

 


Catalog# IW-3012

Unit: Kit

Price: $195

 

The kit components:

  • Picro-sirius Red Solution - 250 ml

  • Acetic Acid Solution - 250 ml

  • Weigert’s Hematoxylin Solution - 250 ml

NovaUltraTM Sudan Black B Lipid Stain Kit

NovaUltra Sudan Black B Stain Kit

This kit is for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections. The lipid and fat will be stained blue-black and cell nuclei will be stained red.

 


Catalog# IW-3021

Unit: Kit

Price: $195

 

The kit components:

  • Sudan Black B Solution - 100 ml

  • Pre-Stain Solution - 100 ml

  • Differentiation Solution - 100 ml

  • Nuclear Fast Red Solution - 100 ml

NovaUltraTM Toluidine Blue Stain Kit

Mast cells are found in the connective tissue and their cytoplasm contains granules (metachromatic) composed of heparin and histamine. This kit is designed for staining of formalin fixed, paraffin embedded tissue sections. Mast cells will be stained dark blue/red purple (metachromatic staining) and the background will be stained light blue (orthochromatic staining).

 


Catalog# IW-3013

Unit: Kit

Price: $125

 

The kit components:

  • Toluidine Blue Stock Solution -25 ml

  • Sodium Chloride Solution - 225 ml

NovaUltraTM von Kossa Stain Kit

This technique is for demonstrating deposits of calcium or calcium salt so it is not specific for the calcium ion itself. In this method, tissue sections are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and thereby visualized as metallic silver.

 


Catalog# IW-3014

Unit: Kit

Price: $195

 

The kit components:

  • Silver Nitrate Solution -100 ml

  • Sodium Thiosulfate Solution - 100 ml

  • Nuclear Fast Red Solution - 100 ml



The following special stain kits are also available upon request.
  • Acid Fast Bacteria (AFB) Stain Kit: The Acid Fast Bacteria (AFB) Stain Kit is intended for use in the histological visualization of Acid Fast Bacteria and Tubercle Bacilli.
  • Alcian Blue (pH 1.0) Stain Kit: The Alcian Blue (pH 1.0) Stain Kit is intended for use in the histological visualization of strongly sulfated mucosubstances.
  • Bielschowsky's Stain Kit (Modified): Bielschowsky's Stain Kit is designed for histological visualization of nerve fibers, neurofibrillary tangles and senile plaques in Alzheimer's disease.
  • Copper Stain Kit (For Microwave): The Copper Stain Kit (For Microwave) is intended for the demonstration of copper deposits in tissue sections.
  • Colloidal Iron Stain Kit: The Colloidal Iron Stain Kit is designed for the histological visualization of acid mucopolysaccharides.
  • Elastic Stain Kit (Modified Verhoff’s): The Elastic Stain Kit is intended for use in histological demonstration of elastin in tissue sections.
  • Eosinophil – Mast Cell Stain Kit: The Eosinophil - Mast Cell Stain Kit is intended for simultaneous visualization of eosinophils and mast cells.
  • Fite’s Stain Kit (Leprosy & Nocardia Procedures): The Fite’s Stain Kit (For Leprosy) is intended for use in the histological visualization of mycobacterium leprae (leprosy).
  • GMS Stain Kit: The Modified Gomori Methenamine-Silver Nitrate Stain (GMS Stain Kit) is intended for use in the histologic visualization of fungi, basement membrane and some opportunistic organisms such as Pneumocystis carinii.
  • Giemsa Stain Kit (May-Grunwald): The Giemsa Stain Kit (May-Grunwald) is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms.
  • Gram Stain Kit: The Gram Stain Kit is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria.
  • Gram Stain Kit (Modified Brown & Brenn): The Gram Stain Kit is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria.
  • H. Pylori Rapid Stain Kit: The H. Pylori Rapid Stain Kit is designed for demonstrating Helicobacter Pylori infected tissue.
  • Jones Stain Kit: Jones Stain Kit is intended for use in histological demonstration of the basement membrane and reticular fibers. This procedure is ideal for staining renal glomerular basement membranes.
  • Movat Pentachrome Stain Kit (Modified Russell-Movat): The Movat Pentachrome Stain Kit (Modified Movat) is intended for use in histological demonstration of collagen, elastin, muscle, mucin and fibrin in tissue sections.
  • Mucicarmine Stain Kit (Modified Southgates’s): Mucicarmine Stain Kit (Modified Southgate’s) is intended for use in the histological visualization of acid mucopolysacharides in tissue sections.
  • PTAH Stain Kit for Microwave (Phosphotungstic Acid Hematoxylin): The PTAH Stain Kit for Microwave is intended for use in the histological visualization of collagen, striated muscle, glial fibers and collagen without using Zenker’s Fixative with Mercuric Chloride as a mordant.
  • Reticulum Stain Kit (Modified Gomori’s): The Reticulum Stain Kit (Modified Gomori’s) is intended for use in histological demonstration of reticular fibers.
  • Pneumocystis Stain Kit: The Pneumocystis Stain Kit is intended for use in the histological visualization of Pneumocystis carinii in cytology smears, and paraffin or frozen tissue sections.
  • Steiner Stain Kit (For Spirochetes): The Steiner Stain Kit (For Spirochetes) is designed for demonstrating Fungi, Helicobacter Pylori, Legionella pneumophila, and Spirochete infected tissue.
  • Warthin-Starry Stain Kit: The Warthin-Starry Stain Kit is intended for use in the visualization of Spirochetes, Helicobacter pylori, Legionella pneumophila, and Cat Scratch Fever bacteria.
  • Wright-Giemsa Stain Kit: Wright-Giemsa Stain Kit is intended to be used for differential staining of blood smears, bone marrow and blood parasites.

 

How to Order

Related Products

 

 

References

 

1. Horiuchi M, et al. Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia. J Neuroinflammation. 2012; 9: 227. Published Online.

 

2. Blume C, et al. Autoimmunity in CD73/Ecto-5′-Nucleotidase Deficient Mice Induces Renal Injury. PLOS One. 2012; 7(5): e37100. Published Online.

 

3. Sun H, et al. AAV-mediated Netrin-1 overexpression increases peri-infarct blood vessel density and improves motor function recovery after experimental stroke. Neurobiol Dis. 2011 October; 44(1): 73–83. Published Online.