Methods & Techniques
For Histologists and Immunohistochemists		  

Antibody Amplifier

 

The Antibody Amplifier™, manufactured by ProHisto, utilizes a common technique used in western blotting to overcome the typical problems that occur in IHC, mainly uneven staining, lack of reproducibility, and high antibody costs. The introduction of the patent-pending Antibody Amplifier™ and Antibody Amplifier Eclipse™ have revolutionized IHC by combining techniques used in western blotting and applying them to IHC.

 

Video Demo Antibody Gallery Antibody Amplifier Meth Protocols

 


Anti-p53 at 1:1,000,000 dilution on mouse colon paraffin section
Anti-p53 at 1:1,000,000 dilution on mouse colon paraffin section
Anti-Ki67 at 1:1,000,000 dilution on mouse colon paraffin section

 

 

How to Order

  • Contact us at sales@ihcworld.com for ordering details. Please indicate the product name, catalog# and quantity.

 

Antibody Amplifier

Catalog No. IW-AA1
Unit: Each
Price:  $65
 Description: One unit is one Antibody Amplifier consisting of 12 compartments, each fitting one standard microscope slide.
The Antibody Amplifier™ utilizes a common technique used in western blotting and applies it to IHC. During antibody incubation, slides are submerged and rocked in a greatly diluted antibody solution. Antibody dilutions at 1:1,000,000 are frequently seen using the Antibody Amplifier™.
Antibody Amplifier Eclipse

Catalog No.: IW-AA2

Unit: Each
 Price:  $75
 Description: One unit is one Antibody Amplifier consisting of 12 compartments, each fitting one standard microscope slide.

 
The Antibody Amplifier Eclipse offers all the benefits of the Antibody Amplifier plus the security of super opaque construction for all your fluorescence needs.The Antibody Amplifier Eclipse™ even improves the ease and confidence of live cell experiments.
Amplifying Antibody Dilution Buffer

Catalog No. IW-AA3
Unit: 500 ml
Price: $50
Description: High quality dilution buffer for primary and secondary antibodies during immunohistochemistry and immunofluorescence.
ProHisto now offers an affordable high quality buffer to use during IHC experiments. This buffer can be used to dilute both primary and secondary antibodies. There is no preservatives to interefere with key enzymes, such as peroxidases. Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 slides. All results on this website are a consequence of using this buffer.
Amplifying Wash Buffer 20X

Catalog No. IW-AA4
Unit: 500 ml
Price: $75
 Description: A high quality, low cost wash buffer for all your IHC and IF needs.
In addition to the Amplifying Antibody Dilution Buffer, ProHisto now offers the Amplifying Wash Buffer to improve your IHC and IF results. This TBST-based buffer consistently and gently washes your slides.All the results on this website are a result of this wash buffer.
Antibody Amplifier Kit 1

 

Catalog No. IW-AAK-1

Unit: Kit

Price: $175
 Description: One classic Antibody Amplifier + 1 bottle of Amplifying Antibody Dilution Buffer + 1 bottle of Amplifying IHC Wash Buffer.

 
For those new to the ProHisto concept, we offer a variety of kits to get you started. Kit 1 offers the classic Antibody Amplifier+1 bottle of Amplifying Antibody Dilution Buffer 1 bottle of Amplifying IHC Wash Buffer.

 Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 samples, and comes ready-to use. The Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.
Antibody Amplifier Eclipse Kit 2




Catalog No. IW-AAK-2

Unit: Kit

Price: $190

Description: One Antibody Amplifier Eclipse + 1
bottle of Amplifying Antibody Dilution Buffer + 1 bottle of Amplifying IHC Wash Buffer.

 

 The Antibody Amplifier Eclipse is perfect for those investigators new to immunofluorescence, or those in need of better, more consistent results. Each kit contains one ultra-opaque Antibody Amplifier Eclipse + 1 bottle of Amplifying Antibody Dilution Buffer + 1 bottle of Amplifying IHC Wash Buffer.

 Each bottle of Amplifying Antibody
Dilution Buffer is enough for 160 samples, and comes ready-to use. The Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.
Antibody Amplifier/Antibody Amplifier Eclipse Kit 3



Catalog No. IW-AAk-3
Unit: Kit
Price: $245
Description: One classic Antibody Amplifier, + Antibody Amplifier Eclipse +1 bottle of Amplifying Antibody Dilution Buffer + 1 bottle of Amplifying IHC Wash Buffer.
This is the ultimate IHC and immunofuorescence kit. It contains both the Antibody Amplifier, the Antibody Amplifier Eclipse + 1 bottle of Amplifying Antibody Dilution Buffer +1 bottle of Amplifying IHC Wash Buffer. This kit gives your lab many options, from IHC to Immunofluorescence to even live cell experiments.

 Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 samples, and comes ready-to use. The
Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.

 

How to Order

  • Contact us at sales@ihcworld.com for ordering details. Please indicate the product name, catalog# and quantity.