Annexin I IHC Antibody, Ready to Use

 

Annexin I IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1006 9 ml $258 Yes

Description

Annexin I, also knows as lipocortin I (Lipo1), belongs to the family of annexins. These proteins are thought to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins (I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are  dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.

 

Catalog Number

 

IW-PA1006

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Liver, skin, cardiac muscle

 

Cellular Localization

 

Membrane, cytoplasmic or nuclear

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval  (HIER) is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Not tested.

ICC: Suitable

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow, E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81, 1986. .

2. Kovacic RT, Tizard R, Cate RL, Frey AZ, Wallner

BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep 17;30(37):9015-21.

3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.

 

Images

 

Formalin fixed paraffin embedded human skin stained with Annexin I antibody showing cytoplasmic staining pattern.

 

Formalin fixed paraffin embedded mouse skin stained with Annexin I antibody showing nuclear staining pattern. 

Immunohistochemical analysis of formalin fixed paraffin-embedded rat cardiac muscle tissue section using Annexin I antibody.

 

 

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