Description

Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD (ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.

Catalog Number

IW-PA1007

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human Annexin IV, different from the related rat and mouse sequence by single amino acid.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Rat cardiac muscle

Cellular Localization

Membrane, cytoplasmic.

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Proteinase K digestion is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Tait, J. F.; Smith, C.; Frankenberry, D. A.; Miao, C. H.; Adler, D. A.; Disteche, C. M. Chromosomal mapping of the human annexin IV (ANX4) gene. Genomics 12: 313-318, 1992.

2. Ponnampalam AP, Rogers PA.Cyclic changes and hormonal regulation of annexin IV mRNA and protein in human endometrium.
Mol Hum Reprod. 2006 Nov;12(11):661-9. Epub 2006 Sep 5

3. Zschornig O, Opitz F, Muller M.Annexin A4 binding to anionic phospholipid vesicles modulated by pH and calcium.Eur Biophys J. 2007 Apr;36(4-5):415-24. Epub 2007 Mar 16.

Images

Immunohistochemical analysis of paraffin-embedded sections

Rat cardiac muscle tissue using Annexin IV antibody