Description

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Catalog Number

IW-PA1015

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human calretinin, identical to the related rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Brain, human mammary cancer.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (Cat# IW-1100) is recommended.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Parmentier M.; "The human calbindins: cDNA and gene cloning.";Adv. Exp. Med. Biol. 255:233-240(1989).

2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.;"The mouse calretinin gene promoter region: structural and functional components.";Brain Res. Mol. Brain Res. 49:175-187(1997).

3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.;"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.";Biochemistry 30:656-662(1991).

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer using Calretinin antibody

Immunohistochemistry staining of formalin fixed paraffin embedded rat brain section with Calretinin antibody.