GST-pi (Glutathione S-Transferases pi) IHC Antibody, Ready to Use

 

GSTpi IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1040 9 ml $258 Yes

Description

Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

 

Catalog Number

 

IW-PA1040

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

Rabbit IgG

 

Immunogen

 

A peptide mapping at the C-terminus of  GSTpi of human origin,different from the related mouse sequence by two sequence.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Endometrial Carcinoma, rat spleen

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Acetone fixed frozen sections.

ICC: Acetone fixed cultured cells.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

[1]. Morrow, C. S.; Cowan, K. H.; Goldsmith, M. E. : Structure of the human genomic glutathione S-transferase-pi gene. Gene 75: 3-11, 1989

2. Islam, M. Q.; Platz, A.; Szpirer, J.; Szpirer, C.; Levan, G.; Mannervik, B. : Chromosomal localization of human glutathione transferase genes of classes alpha, mu and pi. Hum. Genet. 82: 338-342, 1989.

3. Konohana, A.; Konohana, I.; Schroeder, W. T.; O'Brien, W. R.; Amagai, M.; Greer, J.; Shimizu, N.; Gammon, W. R.; Siciliano, M. J.; Duvic, M. :Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. J. Invest. Derm. 95: 119-126, 199.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded human endometrial carcinoma using GSTpi antibody showing cytoplasmic staining. 

Immunohistochemical analysis of formalin fixed paraffin-embedded rat spleen using GSTpi antibody showing cytoplasmic staining. 

 

 

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