ITGA4 (Integrin alpha 4) IHC Antibody, Ready to Use

 

ITGA4 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1046 9 ml $258 Yes

Description

The integrin family includes cell surface receptors for extracellular matrix components as well as receptors involved in various aspects of leukocyte adhesion. Integrin alpha chain 4 (ITGA4) is assigned to 2q31-q32. Interaction between leukemic-cell VLA-4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia.

 

Catalog Number

 

IW-PA1046

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide corresponding to the C-terminal of human ITGA4, identical to the related rat sequence.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Human mammary cancer, human intestinal cancer, HT-1080 cell.

 

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Fernandez-Ruiz, E.; Pardo-Manuel de Villena, F.; Rubio, M. A.; Corbi, A. L.; Rodriguez de Cordoba, S.; Sanchez-Madrid, F. : Mapping of the human VLA-alpha-4 gene to chromosome 2q31-q32. Europ. J. Immun. 22: 587-590, 1992.

2. Jaspers, M.; Zhang, Z.; Marynen, P.; Vekemans, S.; Aly, M. S.; Cuppens, H.; Hillicker, C.; Cassiman, J.-J. : Localization of the genes encoding the alpha-2 and alpha-4 subunits of the human VLA-receptors to chromosome 5q23-31 and 2q31-32 respectively. (Abstract) Cytogenet. Cell Genet. 58: 1870 only, 1991.

3. Zhang, Z.; Vekemans, S.; Aly, M. S.; Jaspers, M.; Marynen, P.; Cassiman, J.-J. : The gene for the alpha-4 subunit of the VLA-4 integrin maps to chromosome 2q31-32. Blood 78: 2396-2399, 1991.

4 Matsunaga, T.; Takemoto, N.; Sato, T.; Takimoto, R.; Tanaka, I.; Fujimi, A.; Akiyama, T.; Kuroda, H.; Kawano, Y.; Kobune, M.; Kato, J.; Hirayama, Y.; Sakamaki, S.; Kohda, K.; Miyake, K.; Niitsu, Y. : Interaction between leukemic-cell VLA-4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia. Nature Med. 9: 1158-1165, 2003. Note: Erratum: Nature Med. 11: 578 only, 2005.

 

Images

 

Immunohistochemical analysis of paraffin-embedded sections

Human mammary cancer using ITGA4 antibody

Staining ITGA4 in cytoplasm. DAB chromogenic reaction.

 

Immunocytochemistry analysis of HT-1080 cell using ITGA4 antibody

 

Immunohistochemical analysis of paraffin-embedded sections

Human intestinal cancer using ITGA4 antibody

 

 

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