MAPK 1/3 IHC Antibody, Ready to Use

 

MAPK 1/3 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1049 9 ml $258 Yes

Description

MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF  at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.

 

Catalog Number

 

IW-PA1049

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide mapping at the N-terminal of the human MAPK1+3, identical to the related rat sequence.

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Human intestinal cancer

 

Cellular Localization

 

Cytoplasmic/nuclear

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Bhalla, U. S.; Ram, P. T.; Iyengar, R. : MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Science 297: 1018-1023, 2002.

2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein kinases. Oncogene 9: 647-649, 1994.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded human intestinal cancer using MAPK1-3 antibody. Staining MAPK1-3 in cytoplasm and nucleus. DAB chromogenic reaction.

 

 

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