Bcl-2 IHC Antibody, Ready to Use

 

Bcl-2 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-MA1004 9 ml $258 Yes

Description

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

 

Catalog Number

IW-MA1004

 

Quantity

9 ml

 

Host

Mouse

 

Clone

BL-2

 

Isotype

Mouse IgG1

 

Immunogen

Synthetic peptide corresponding to residues 41-54pf the bcl-2 protein, conjugated to thyroglobulin

 

Purity

Purified by the goat anti-mouse IgG affinity chromatography

 

Conjugate

Uconjuated

 

Species Reactivity

Human. Not tested in other species

 

Positive Control

Human tonsil

 

Cellular Localization

Membrane/cytoplasmic.

 

Form

Ready to use solution in PBS based antibody dilution buffer containing 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted

 

Storage

Store at 2-8 °C. Do not freeze.

 

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Formalin fixed or acetone fixed frozen sections.

ICC: Suitable.

 

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

1. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t(14;18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988.

2. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990

 

Images

Immunohistochemical staining of formalin fixed paraffin-embedded human tonsil using Bcl-2 antibody. DAB chromogen substrate.

 

 

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