p53 IHC Antibody, Ready to Use

 

p53 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-MA1078 9 ml $258 Yes

Description

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

 

Catalog Number

IW-MA1078

 

Quantity

9 ml

 

Host

Mouse

 

Clone

IMD-53

 

Isotype

Mouse IgG2a

 

Immunogen

Recombinant human wild-type p53 protein.

 

Purity

Purified by the goat anti-mouse IgG affinity chromatography.

 

Conjugate

Unconjugated

 

Species Reactivity

Human. Not tested in other species.

 

Positive Control

Human skin, tumor.

 

Cellular Localization

Nuclear

 

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

Store at 2-8 °C. Do not freeze.

 

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Formalin fixed or acetone fixed frozen tissues.

ICC: Suitable.

 

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

1. Isobe, M.; Emanuel, B. S.; Givol, D.; Oren, M.; Croce, C. M. : Localization of gene for human p53 tumour antigen to band 17p13. Nature 320: 84-85, 1986.

2. Casey, G.; Yamanaka, Y.; Freiss, H.; Kobrin, M. S.; Lopez, M. E.; Buchler, M.; Beger, H. G.; Korc, M. : p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. Cancer Lett. 69: 151-160, 1993.

 

Images

 

Immunohistochemical staining of formalin fixed paraffin-embedded human intestine cancer using p53 antibody showing nuclear staining pattern. DAB chromogen substrate. 

 

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