Polink-DS (Double Staining) IHC Detection System

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Polink DS System

Polymer-HRP and Polymer-AP Double Staining System for

Mouse and Rabbit Antibodies

Tonsil tissue double stained by Ms. Anti-B cell and Rb anti-CD3 using

Polink DS kit (Cat no. D20-6A)

Features

Biotin-free double staining detection system
Avoid cross-reactions of sequential double staining
Simultaneous incubation of primary antibodies
Simultaneous incubation of secondary antibodies
4 step method compared to sequential 14 step method
Two formats:

Polink DS-MR kit is designed to simultaneously detect two different antigens at different locations within the same tissue section. It utilizes proprietary high sensitivity polymer-HRP and polymer-AP technology. The primary antibodies may be rabbit and mouse.

Comparing to conventional double staining methods, Polink DSMR kit dramatically reduces the staining steps from 14 steps to 4 steps. Polink DS-MR kit overcomes the cross-reactions due to insufficient blocking of conventional sequential Biotin-Strepativdin/Alkaline Phosphatase double staining method.

We offer two different formats for double staining: Polymer-HRP x Mouse with polymer-AP x Rabbit and polymer-HRP x Rabbit with polymer-AP x Mouse. Refer to the following table to choose double staining with brown color and red color (DAB + Fast Red) or red color and purple color (AEC + BCIP/NBT).

Catalog No.	Product Name	Enzyme Conjugate & Primary Antibody	Chromogen
D20-6A	Polink DS-MR w DAB/AP-Red+, 6 ml	HRP x Mouse and AP x Rabbit	DAB & AP-Red+
D20-6F	Polink DS-MR w DAB/Fast Red, 6 ml	HRP x Mouse and AP x Rabbit	DAB & Fast Red
D63-6	Polink DS-MR w BCIP/AEC, 6 ml	HRP x Mouse and AP x Rabbit	AEC & BCIP/NBT
D32-6F	Polink DS-MR w DAB/Fast Red, 6 ml	HRP x Rabbit and AP x Mouse	DAB & Fast Red
D64-6	Polink DS-MR w BCIP/AEC, 6 ml	HRP x Rabbit and AP x Mouse	AEC & BCIP/NBT

Polink DS System

Polymer-HRP and Polymer-AP Double Staining System for

Two Mouse or Two Rabbit Antibodies

Rabbit x Desmin (brown) and rabbit x CEA (Red) stained by D79-6F on colon cancer

Features

Biotin-free double staining detection system
Total of 7 steps compared to traditional 14 steps
Can be used for double staining of two antibodies from the same species
Two formats:

Polink DS-MM kit and Polink DS-RR kit is designed to detect two different antigens at different locations within the same tissue section. The primary antibodies may be two mouse antibodies or two rabbit antibodies. It utilizes GBI’s proprietary DS Blocker to effectively prevent the second staining from interfering with the first staining.

Compared to conventional double staining methods, Polink DS kit dramatically reduces the staining steps from 14 steps to 7 steps. Polink DS kit overcomes the cross-reactions due to insufficient blocking of sequential Biotin-Strepativdin/Alkaline Phosphatase double staining method.

We offer two different formats for double staining: Polymer-HRP anti Mouse/polymer-AP anti Mouse and polymer-HRP anti Rabbit/polymer-AP anti Rabbit. Refer to the following table to choose double staining with brown color and red color (DAB + Fast Red or AP-Red) or red color and purple color (AEC + BCIP/NBT).

Catalog No.	Product Name	Enzyme Conjugate & Primary Antibody	Chromogen
D78-6F	Polink DS-MM w DAB/Fast Red, 6 ml	HRP x Mouse and AP x Mouse	DAB & Fast Red
D78-6A	Polink DS-MM w DAB/AP-Red+, 6 ml	HRP x Mouse and AP x Mouse	DAB & AP-Red+
D79-6	Polink DS-MM w BCIP/AEC, 6 ml	HRP x Mouse and AP x Mouse	BCIP/NBT & AEC
D80-6F	Polink DS-RR w DAB/Fast Red, 6 ml	HRP x Rabbit and AP x Rabbit	DAB & Fast Red
D80-6A	Polink DS-RR w DAB/AP-Red+, 6 ml	HRP x Rabbit and AP x Rabbit	DAB & AP-Red+
D81-6	Polink DS-RR w BCIP/AEC, 6 ml	HRP x Rabbit and AP x Rabbit	BCIP/NBT & AEC

How to Order

PO can be Emailed to sales@ihcworld.com or Faxed to 443-638-0394
Credit Card Order need to call us at 443-686-9618
Online Order can be placed on our Online Store at www.ihcworld.com/catalog/