ABC of Safety in the Biological Sciences
TOLUIDINE BLUE (Colour Index No. 52040) (C15H16N3SCl)
Methylene blue T 50, methylene blue T extra, tolonium chloride, blutene chloride, blutene, basic blue 17.
Animal mutagen not to be handled by staff who are pregnant. Avoid skin and eye contact.
Keep away from heat.
Keep away from oxidising materials.
Wash hands thoroughly after handling.
Black/green or black/brown, combustible powder. Quinoneimine (thiazin) group of dyes.
HEALTH HAZARD DATA
This is a combustible substance and should never be handled close to heat or a naked flame. It is an animal mutagen and should not be handled by staff who are pregnant. It is an eye irritant. Its toxicity has not been quantified but has proven to be highly toxic to laboratory animals when injected intravenously. Other dyes in this group are known to be highly toxic, e.g. methylene blue.
Skin remove contaminated clothing then wash the affected area with soap or mild detergent and large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek medical advice. Wash contaminated clothing before reuse.
Eyes immediately wash the affected eye with large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek immediate medical attention.
Inhalation remove from the area of exposure to fresh air. If breathing has ceased apply artificial respiration. Give oxygen if required. Keep warm and allow to rest. Seek immediate medical attention.
Ingestion wash out the mouth thoroughly with water and seek immediate medical attention.
Heating produces toxic fumes of chlorine, sulphur and nitrogen compounds. Can react with oxidising materials. This is a combustible substance and should never be handled close to heat or a naked flame.
Store in a cool, dry atmosphere away from heat or oxidising materials.
Use a fume hood to keep the level of exposure to a minimum. Wear protective clothing. A long sleeved laboratory coat or gown, rubber gloves, safety goggles and a face mask as a minimum standard.
Butyl rubber gloves, laboratory coat and self contained breathing apparatus.
On skin and clothing: wash skin with strong soap solution immediately. Rinse thoroughly. Contaminated clothing should be removed, dried and washed with strong soap solution or destroyed. It may be necessary to destroy shoes by burning.
Small spills: absorb liquid on paper towels. Brush solids on to paper. Place in an iron pan and allow evaporation in a fume hood. Add crumpled paper and burn. Wash spill site with a strong soap solution.
Large spills: cover with sand and soda ash mixture (90/10). Mix and shovel into a cardboard box. Pack with a large excess of crumpled paper. Burn in an open pit or incinerator equipped with an afterburner and scrubber.
PACKAGE LOTS: Either
1 Pour or sift on to a thick layer of sand and soda ash mixture (90/10). Mix, and shovel into a heavy paper box with much paper packaging. Burn in an incinerator. The fire may be augmented with scrap wood. Stay on the upwind side.
2 Dissolve in a flammable solvent and spray into the firebox of an incinerator equipped with an afterburner and scrubber.