IMVS Division of Pathology
The Queen Elizabeth Hospital
Woodville Road, Woodville, South Australia 5011
Again this problem is usually associated with incomplete dehydration, clearing and/or impregnation. Most commonly though it is caused by incomplete dehydration. The affected tissue needs to be rehydrated and then re-processed because otherwise these sections do not stain properly. They stain very darkly with both haematoxylin and eosin making a diagnosis very difficult. And they have a marked tendency to float off the slide during staining.
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© Roy C. Ellis 2002