Histology, Immunohistochemistry & TEM Services

 

Dedicated to High Quality & Fast Reliable

Histology, Immunohistochemistry and TEM Services

 

 

Sample Submission Form

 

We Also Offer the Custom Antibody Services

 

Custom Antibody Production Services

 

Antibody Screening Services

 


 

IHC World offers a full range of histology and TEM services including Routine & Customized Histology, Neurohistology, Bone Histology, Histopathology, Tissue Microarray, Immunohistochemistry (IHC), Immunofluorescence (IF, ICC), Apoptosis Detection (TUNEL Staining), Whole Mount IHC, Whole Slide Scanning, Confocal Microscopy and Transmission Electron Microscopy (TEM). We also provide hands-on training, consulting, professional and technical support services. With over 20 years combined academic and industrial experiences, we are confident that IHC World will achieve high quality work and guarantee you complete satisfaction from project design, development to completion. Please feel free to give us a call to request a price quotation or talk with one of our scientists for any questions you may have in mind.

 


 

General Histology Service

  • Paraffin Embedding & Sectioning

  • Cell Pellet Embedding & Sectioning

  • Multi-Tissue Embedding & Sectioning

  • Frozen Embedding & Sectioning

  • H&E Staining

Special Staining Service

  • We offer the following special staining service using our standard protocols: Alcian Blue, Alizarin Red, Bielschowsky, Congo Red, Fontana Masson, Giemsa, Golgi, Gram, Luxol Fast Blue, Masson Trichrome, Methenamine Silver, Microglia, Nissl, Orcein Elastin, Oil Red O, PAS, Prussian Blue, Safranin O, Sirius Red, Toluidine Blue, von Kossa, X-Gal.

  • Please contact us if you need us to develop new staining methods to meet your specific needs.


Luxol Fast Blue

Fontana Masson

Nissl

Oil Red O

PAS

Prussian Blue

Safranin O

Masson Trichrome

von Kossa/Alcian Blue

 

IHC Validation Service

  • In order to successfully and consistently perform routine immunohistochemical staining, it is critical to develop an optimal protocol for a specific antibody. Our IHC Validation Service is designed to validate an antibody and develop a standard staining protocol for a specific antibody therefore reaching the maximal signal to noise ratio.


No AR 1:100

No AR 1:500

No AR 1:1000

PIER 1:100

PIER 1:500

PIER 1:1000

HIER 1:100

HIER 1:500

HIER 1:1000

 

IHC Routine Service

  • We perform routine immunohistochemistry (IHC), immunofluorescence (IF) and immunocytochemistry (ICC) staining using our well-established, optimized antibody staining protocols on formalin fixed paraffin embedded tissue sections, fresh frozen sections, cultured cells and whole mount samples.


CD31

Collagen IV

CA-IX

Filaggrin

p53

Heme Oxygenase-1

Ki67

p-Mek

PCNA

 

IHC Double Label Service

  • We also perform double labeling immunohistochemistry (IHC) and immunofluorescence (IF) services to localize two proteins simultaneously on the same tissue section.


vWF DAB-brown & eNOS AP-red

 MITF red & Tryptase green

Iba1 green & b-Amyloid red

 

Apoptosis Detection Service

  • Apoptosis, also called programmed cell death, is involved in many physiological processes by which unwanted or useless cells are eliminated during development and other normal biological processes. Cells undergoing apoptosis show characteristic morphological and biochemical features. There are many methods involved in the detection of apoptosis. We offer TUNEL staining for the detection of apoptosis in formalin-fixed, paraffin-embedded tissues, cryostat sections, cell suspensions, cytospins, and cell cultures.

  • TUNEL Staining: Label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by specific staining. The end products can be enzyme end labeling or fluorescent end labeling.

 

Whole Mount IHC Service

  • We perform routine Whole Mount IHC staining using standard immunofluorescent and immunoperoxidase methods. We can also help to develop project specific staining protocols according to client's requirements.

   

Brain-Array Service

  • Embedding of multiple brains in one paraffin block

  • Sectioning at 5um, 10um, 25um and 50um thickness depending on your specific need

  • Perform H&E staining, Nissl staining, special staining and IHC staining

  • Imaging analysis and stereology analysis

Advantage: Allow simultaneously processing, sectioning and staining all experimental and control brains under same condition therefore reducing experimental variability.

 

 

Transmission Electron Microscopy (TEM) Service

  • Complete TEM service.

  • TEM thick sectioning and toluidine blue staining.

  • TEM thin sectioning and contrast staining.

  • Negative Staining for viral & bacterial detection & mycoplasma contamination analysis.

  • TEM Immunogold Labeling service for protein sub-cellular localization.


Bacteriophage - Negative Stain

VLP - Negative Stain

 

 

Pathological Evaluation

  • Scoring, reading, analysis and reporting by Board Certified Pathologists.

Image Analysis & Stereology Analysis

  • Digital imaging

  • Image analysis using standard software

  • Stereology analysis for neuroscience research in partnership with Sinq Systems

  • Whole slide scanning and confocal microscopy

Tissue Microarray (TMA) Construction Service

  • TMA is a powerful technology for high throughput analysis of biomarker expression in a large number of tissue samples. These samples include human tissues, animal tissues as well as cell lines. Our TMA Construction Service includes design and creation of customized paraffin recipient blocks, tissue core transfer and insertion, TMA block sectioning and TMA slide staining.

 

Customer Service

Technical Support

General Inquiry

Mailing Address

  • IHC WORLD, LLC

  • P.O. Box 211

  • Woodstock, MD 21163

Shipping Address

  • IHC WORLD, LLC

  • 3225 Corporate Court

  • Ellicott City, MD 21042