Cell Markers Review
Discuss how to identify specific cell types (endothelial cell, macrophage, stem cell, etc) using specific antibodies as detection markers.
Tumor Markers Review
By using antibodies as detection markers, one can identify tumors or disease conditions such as prostate cancer, hypoxia, necrosis, and more.
Immunohistochemistry (IHC)
This forum is for general questions, methods, protocols and tips regarding immunohistochemistry techniques on paraffin, frozen, or free floating tissue sections
Immunocytochemistry (ICC)
This forum is for general questions, methods, protocols and tips regarding immunocytochemistry techniques on cell suspension, cultured cells or cytospin.
Immunofluorescence (IF)
This forum is for general questions, methods, protocols and tips regarding immunofluorescence techniques on paraffin, frozen sections or cultured cells.
Enzyme Linked ImmunoSorbentAssay (ELISA)
technique for detecting the presence of specific substances, such as enzymes, viruses, bacteria, or antibodies in blood.
Flow Cytometry (FC)
analysis of cells or subcellular components by detecting fluorescence or light-scatter of sample fractions passing in narrow-stream droplets through a laser beam.
Western Blotting (WB)
technique which transfers proteins from a gel to a nitrocellulose or nylon membrane following electrophoresis and uses specific antibodies to bind and visualize the protein of interest.
25 Posts
8 Topics
Last post by Welly S
in HIF-1a
on May 06, 2008, 09:01:53 PM
Antigen Retrieval
What is antigen retrieval? What method should I use? Share you questions, answers, protocols and tips here.
General Histology
For questions and answers regarding general histology, including tissue processing, embedding, sectioning, special staining, enzyme staining and silver staining, etc.
Electron Microscopy
EM is not very popular technique, but could be a crucial tool for suporting your research. Any questions and answers about TEM, SEM, negative staining, immuno-EM are welcome.
Apoptosis or Cell Death
Apoptosis, programmed cell death, is among the hottest research fields. Detection methods appear to be important: TUNEL, caspase3, Bcl2, and what else?
Image Analysis
Image analysis, especially stereological analysis, is probably most oftenly used by neuroscientist. Discuss your questions, methods, software used, etc.
Histology Products
Use this forum to discuss histology products (reagents, staining kits, etc.) you plan to purchase or have used in the past. Share your questions, answers, experience, comments, and reviews.
Histology Equipment
Buy, sell, exchange or discuss histology (processor, embedding center, microtome, slide labeler, coverslipper) and immunohistochemistry (manual or autostainer) related equipment and accesseries.
Tumor and Antibody Panel
This place is for discussion about immunoreactivity of various tumors and the antibody panel that can differentiate between tumors
IHC World Forum - Info Center
