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Author Topic: Rabbit polyclonal F-actin (or pan-actin) antibody  (Read 3298 times)

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Offline lyna731

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Rabbit polyclonal F-actin (or pan-actin) antibody
« on: August 24, 2005, 05:12:13 PM »
Hi,

I'm looking for a good rabbit polyclonal anti-F-actin antibody (or pan-actin) for using in immunofluoresence studies.
I would be very gratefur to you if you could tell me your experience.
Thanks very much.
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Rabbit polyclonal F-actin (or pan-actin) antibody
« on: August 24, 2005, 05:12:13 PM »

Offline discman

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Rabbit polyclonal F-actin (or pan-actin) antibody
« Reply #1 on: August 25, 2005, 05:41:08 AM »
Hi,

I used the Sigma D-5060 rabbit polyclonal anti-actin with good results even in tricky tissue (intervertebral disc matrix). Antigen retrievall was done by pepsin digestion of the tissue. 1:100 dilution worked fine (didn't try to go lower though).

Hope this helps,
Günther

PS However, we have problems to get this ab going on WB
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Rabbit polyclonal F-actin (or pan-actin) antibody
« Reply #1 on: August 25, 2005, 05:41:08 AM »
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