We'are trying a new method for quantitation of IgG in dog (cat) serum developed against food compounds. The diluition of dog serum and the respective HRp-conjugated secondary antibody seem the main problems.
May be the buffers of diluition should be different.
What about the addition of a small quantity of BSA (5%), exspecially at the first solution we prepare, that one we use for the dog serum?
Thanks for any suggestion.
