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Offline Leonard Cheung

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Ki 67
« on: December 03, 2005, 07:20:27 AM »
Anyone knows the optimal dilution of mouse anti-rat ki 67 MIB-5 from Dako for skeletal muscle? As I have checked the instruction from Dako website, but it is not available for public now.

Thanks!
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Ki 67
« on: December 03, 2005, 07:20:27 AM »

Offline richard03

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Ki 67
« Reply #1 on: December 03, 2005, 11:04:41 AM »
The following protocol is similar to the antibody you mentioned. Use this a general guideline.

http://www.ihcworld.com/_protocols/antibody_protocols/ki67_dakocytomation.htm

Hope this helps.

Richard
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Offline Leonard Cheung

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Ki 67
« Reply #2 on: December 03, 2005, 01:16:07 PM »
thx Richard,

I also have looked this protocol before, but I worry that the dilution is different because of different species.
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Offline richard03

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Ki 67
« Reply #3 on: December 03, 2005, 01:24:18 PM »
Leonard,

I just checked Dako website and found the datasheet. The recommended dilution is 1:25-1:50 and AR is citrate buffer pH6. Here you go.

http://www.dakousa.com/prod_downloadpackageinsert.pdf?objectid=112400001
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Offline Leonard Cheung

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Ki 67
« Reply #4 on: December 03, 2005, 02:25:30 PM »
O.... Thanks a lot!
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Offline JennW098

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Re: Ki 67
« Reply #5 on: January 04, 2007, 09:20:14 PM »
Hi -- for what it's worth, Abcam has a Ki67 rabbit mono-clonal (yes, unusual to have a rabbit mono-clonal) which works beautifully in rat using the DAKO Envision kit which is easy as pie.  Good luck with it!
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Offline ImmunoNYC

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Re: Ki 67
« Reply #6 on: January 05, 2007, 10:47:08 AM »
I have in my notes I have used MIB5 at 1-3 ug/ml with DAKO's Target Retrieval Solution with good success. Good luck and don't forget to come back and let us know how it works out.
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Re: Ki 67
« Reply #6 on: January 05, 2007, 10:47:08 AM »
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