Baclight is probably a great product if you were just staining cultures or smears. However, you say you are staining chronic wounds. Chronic wounds will contain live and dead bacteria as well as mostly dead tissue, of which the cells have lysed and could also pick up staining. Necrotic tissue is notorious for this.
Are there RBCs and WBCs present? Are you staining a tissue sample of the wound or a swabbing? Were the wounds non-healing and wet or healing and dry? What fixative are you using? How thick are the sections? Have you performed a Gram stain on any sections?
