Author Topic: 3d cell culture embedding  (Read 2988 times)

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Offline JennW098

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3d cell culture embedding
« on: January 04, 2007, 09:25:15 PM »
Hi -

Has anyone done 3d cell culture and then tried to embed in LR White?  I was so frustrated when I found that my perfect sample started to fall apart when I tried to cut it and remove it from the dish to put in empty capsules.  I did also try coating the top with agarose (low melt) but that did not help.  Any ideas?  It's in LR White at -4C right now.  First I just want to embed and cut for Light Microscopy, but of course the ultimate goal is ultrastructure with EM.

EM experts, please help!!

jw

3d cell culture embedding
« on: January 04, 2007, 09:25:15 PM »

Offline excalibur

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Re: 3d cell culture embedding
« Reply #1 on: January 05, 2007, 10:16:13 AM »
Do you have any of the tiny silicon EM molds that are flat instead of Beem capsules? They will help with placing your specimen at the tip with less manipulation.
Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
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Offline daryy

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Re: 3d cell culture embedding
« Reply #2 on: March 06, 2009, 08:45:02 PM »
Parsons said at her Blush Bridal, they have a variety of sizes and price points for every bride.
Back in her bridal suite, after trying on flowy wedding dresses after dress, Cole tried just one more. A lace cinderella wedding dresses with a keyhole back.
It's exactly what she's been looking for. "I've found nothing that comes close to the feeling I have when this is on."
On this day, Cole said yes to her dress.
"As soon as I got the allure wedding dresses, now everything kind of falls into place."
I faced facts a long time ago: I'm never going to be a fashion designer. Whatever that special something amazing is that the greats like Yves Saint Laurent, Alexander McQueen, and Coco Chanel have, I just don't got it.
But I still love fashion. It inspires the things I wear and the things I display in my home as well as all of my work as a wedding and event designer.
Of course my love extends to wedding and bridal fashion, too. I've been designing weddings for over thirty years, so I've worked with a lot of brides. Designers have to know their clients, and, for me, that means knowing what trends my brides are excited about. What are the patterns, cuts, materials and embellishments they can't get enough of? Because if a bride loves a certain detail on her trumpet wedding dresses, she'll probably love it, or something similar, in her bouquets and table centerpieces and even on her invitations.
Naturally, I try and follow New York Bridal Fashion Week as closely as I can, and this last week I've fallen in love with so many of the fall 2012 designs. The new gowns are just that, gowns. They're big and beautiful with lots of texture and color. Simplicity is out, and I couldn't be happier!
I love that Vera Wang didn't shy away from drama. Her models walked the runway in beige, grey and even black modest wedding dresses. But not one of them wore a white gown! Personally, I loved her grey and beige wedding dresses sash with lace overlay and tulle the most. They're elegant and sophisticated without feeling familiar.
Vera wasn't the only designer who added some color to the catwalk, though. Pink was everywhere: JLM Couture, Anna Maier, Reem Acra, Anne Barge, Alfred Angelo and Ines Di Santo all showed off beautiful pink and blush-colored gowns.
As a designer I absolutely love color. Color makes weddings and every other special event feel more alive. Unfortunately, some brides are hesitant about incorporating too much color into their wedding day style and decor. But I'm hoping these colorful new wedding dresses will make them reconsider. After all, if you love color in your every day life, there's no reason you shouldn't have lots of color on your wedding day, too.
It was Reem Acra's collection, though, that truly spoke right to my heart; the new backless wedding dresses are rich with embellishments! The gowns feature intricate beading, feathers, flowers, embroidered bodices, illusion sleeves and big, elaborate skirts. This collection doesn't take itself too seriously -- every colored wedding dresses feels whimsical and fun but also glamorous.
What I love most about these two trends -- color and embellishments -- is how easy they are to replicate. So although Vera Wang and Reem Acra, for example, are fabulous, you don't have to wear one of their gowns to get the look.
And if you love color but also love the idea of wearing that big white gown, you can do both! Consider asking your seamstress to sew your favorite colorful fabric to your sweetheart wedding dresses' crinoline petticoat; as you walk down the aisle everyone will catch glimpses of your favorite color beneath your long white gown. You could also sew a bright, colorful border to the outer edge of your classic wedding dresses. Or, attach a bright bow with long ribbons to your waist so that strips of color cascade down the back of your organza wedding dresses.
The same is true for embellishments. Feathers and fabric flowers, especially, are inexpensive and easy to work with; it only takes a few well-placed feathers to make a big statement.
I always ask my brides to show me their vintage wedding dresses; it's a great way for me to get an immediate sense of their style and how they envision their wedding day. If a bride's mermaid wedding dresses has some intricate beading, hints of color, bows, flowers, feathers, ribbons or unique textures, I try to incorporate a similar look and feel into my designs.
But you don't have to be a designer to pull this off.
Think about adding a few of these complementary details to your wedding invitation suite. What about a sparkly border, or using card stock that has a subtle shimmer? Or, use a wax seal on your envelopes in the shape of a feather, bow or the flower pattern on your one-shoulder wedding dresses.
I love using these details in my table linens, too. If your halter wedding dresses or wedding color palette features shades of pink, for example, look for linens with a soft pink sheen. If you're wearing a ribbon belt, think about adding a matching ribbon edge to your linens or the back of your reception chairs.
And if you're making your own bouquet, wrap your flowers in a matching ribbon or bow. You can even tuck sparkly beads or Bridal Gowns between the greenery and flowers in your bouquet for a bit of unexpected glamour. For many more ways to incorporate feathers into your wedding day style check out my last post.

« Last Edit: November 19, 2011, 08:47:06 PM by daryy »

Re: 3d cell culture embedding
« Reply #2 on: March 06, 2009, 08:45:02 PM »