new to cytochemistry

[D_Errington]

Well, I have done just about every other type of histology, and now have been asked to prepare some immuno-Cyto slides...

I have one question...and please forgive my ignorance on the subject....

What side of the cover slips will the cells grow on?
Do I keep the cover slip in it's original orientation when mounting, or do I have to flip it over (my guess is that they grow on the top of the slide while it is in the cell culture plate)

thanks, I have searched every protocol I can find and none of them have mentioned slide/cell orientation:)

[gabor]

hi,
really i don't understand the way you make cells growing on the cover slip (alias slide?) . Normally  I utilized specifical slides with 8 wells  divided by a plastic grid-barrier, to permit a right orientated growing of adherent cells and to allow different stainings in the same slide.


http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/C6432

[D_Errington]

yes, they are grown in 6-well cell culture plates.
I have already run the protocol once and will find out which orientation works best today.

[science]

You would flip the coverslip so that the cells lie between the glass slide and the coverslip . . . like a sandwich.  This will prevent the cells from becoming dislodged.

[daryy]

if you know more about the cells! You will  get more interest from it!