Pancreatic cells in mouse kidney section ??

[kuno]

Hi people,

I transplanted human stem cells into the kidney capsule and retrieve them 2 months later. The H&E slides showed graft like structure which look like exocrine/endocrine. My supervisor told me that these cells could be originated from the mouse it self (mouse pancreatic tissues which attached to the kidney).

As i'm not an expert in mouse histology/anatomy, I would like to ask people opinion in the forum. Is it common for the mouse pancreas to be attached to the kidney??? Cause i'm pretty sure that we separate the kidney from other tissues physically.

I'm planning to do human specific Antibody staining as well in the future.

Thank you

Daniel  Lie
PhD Student
Diabetes Transplant Unit

[kuno]

Another question for people out there,

Can I used Goat secondary Antibody for primary antibody raised in sheep??

Thank you

Daniel Lie

[richard03]

Yes. you can use Goat anti-sheep for primary ab raised in sheep.

Another question for people out there,

Can I used Goat secondary Antibody for primary antibody raised in sheep??

Thank you

Daniel Lie

[ImmunoNYC]

Kidney and pancreas are quite separate. Are the cells you see under the kidney capsule? Can you load some photos? Are you sure it isn't adrenal which is often taken with kidney.

Why not stain for human and mouse specific cytokeratins, MHC or DNA to see which cell type they are.

You can use goat anti-sheep IgG to detect a sheep primary just make sure that if you use it on mouse tissue sections it is preadsorbed against mouse IgG.