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Author Topic: Gene knockdown in bacteria  (Read 8098 times)

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Offline imani_a

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Gene knockdown in bacteria
« on: December 02, 2008, 12:08:24 AM »
Hi Everbody
my question about How can I insert SiRNA in gram negative bacteria?
In Eukaryote cell by transfectamin is possible but in prokaryote cell(bacteria) is impossible
please let me methods os insertion SiRNA in bacteria
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Gene knockdown in bacteria
« on: December 02, 2008, 12:08:24 AM »

Offline ImmunoNYC

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Re: Gene knockdown in bacteria
« Reply #1 on: February 14, 2009, 01:34:06 PM »
So not my forte but would regular transformation work?
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Offline ImmunoNYC

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Re: Gene knockdown in bacteria
« Reply #2 on: February 14, 2009, 01:36:19 PM »
I know nothing about bacteria but are the mechanics of RNAi conserved in bacteria? Have you looked in literature, someone must have done what you are trying to do?
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Re: Gene knockdown in bacteria
« Reply #2 on: February 14, 2009, 01:36:19 PM »
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