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Author Topic: Staining brain tissue that was never frozen  (Read 1243 times)

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Offline Thitus

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Staining brain tissue that was never frozen
« on: August 29, 2009, 06:01:17 PM »
Hi,

I received human brain tissue, which was perfused with PFA and kept in it for several years. It was never frozen. I wonder what the best sectioning procedure would be.

Can I still cryoprotect the tissue in 20% and 30% sucrose and do cryostat sectioning or should I use a vibratome?

Does too long postfixation impair cryoprotection?
« Last Edit: August 29, 2009, 06:18:45 PM by Thitus »
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Staining brain tissue that was never frozen
« on: August 29, 2009, 06:01:17 PM »

Offline richard03

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Re: Staining brain tissue that was never frozen
« Reply #1 on: August 29, 2009, 08:04:28 PM »
processing, embedding and sectioning procedures should no difference. The brain tissue should work fine for routine histology and special staining. But it may not work well for immunohistochemistry.
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Offline gula

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Re: Staining brain tissue that was never frozen
« Reply #2 on: August 31, 2009, 11:58:05 AM »
It depends on your following testing, but why not embed in paraffin? If you want to stain the brain with procedures, that also work on ffpe-tissue, I would prefer the better morphology of a paraffin-section.
gula
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Re: Staining brain tissue that was never frozen
« Reply #2 on: August 31, 2009, 11:58:05 AM »
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