Help for spleen frozen section

[Lucyusf]

Hi, everybody,

I'm new to spleen section. I tried same thing as brain sectioning. 4% paraformadehyde 24h, 10% sucrose 24h, 20% sucrose 24h and 30% sucrose 24h, then cutting at -20 degree. Unfortunately, I got a lot of brown thing on the slide even with hematoxylin staining. I tried to attach my picture, but it didn't work.

Can anybody help me with the problem?

Thank You very much.

Lucy

[excalibur]

Spleen is bloody and shatters easily, even in paraffin. Try cutting warmer or holding your thumb on the block face a sec and then quickly cut.

[Lucyusf]

Thanks a lot. I'll try to increase the temperature.

Lucy

[Lucyusf]

Attached is the spleen stained with hematoxylin alone.

[chicago]

Spleen is bloody and shatters easily, even in paraffin. Try cutting warmer or holding your thumb on the block face a sec and then quickly cut.