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Author Topic: Help for spleen frozen section  (Read 609 times)
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Lucyusf
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« on: September 23, 2009, 10:34:48 AM »

Hi, everybody,

I'm new to spleen section. I tried same thing as brain sectioning. 4% paraformadehyde 24h, 10% sucrose 24h, 20% sucrose 24h and 30% sucrose 24h, then cutting at -20 degree. Unfortunately, I got a lot of brown thing on the slide even with hematoxylin staining. I tried to attach my picture, but it didn't work.

Can anybody help me with the problem?

Thank You very much.

Lucy
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excalibur
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« Reply #1 on: September 23, 2009, 11:16:13 AM »

Spleen is bloody and shatters easily, even in paraffin. Try cutting warmer or holding your thumb on the block face a sec and then quickly cut.
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Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway
Moore, OK 73160
405-759-3953
contact@excaliburpathology.com
www.excaliburpathology.com
Lucyusf
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« Reply #2 on: September 24, 2009, 05:49:11 AM »

Thanks a lot. I'll try to increase the temperature.

Lucy
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Lucyusf
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« Reply #3 on: September 28, 2009, 09:03:26 AM »

Attached is the spleen stained with hematoxylin alone.
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chicago
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« Reply #4 on: January 29, 2010, 06:11:52 AM »

Spleen is bloody and shatters easily, even in paraffin. Try cutting warmer or holding your thumb on the block face a sec and then quickly cut.
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