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Author Topic: Immunostaining  (Read 1019 times)

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Offline Lee

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Immunostaining
« on: November 09, 2009, 02:05:25 PM »
Can frozen tissue that has been immunostained be immunostained again with a different antibody after the first immunostaining is finished?  If so, what steps need to be taken?
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Immunostaining
« on: November 09, 2009, 02:05:25 PM »

Offline excalibur

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Re: Immunostaining
« Reply #1 on: November 11, 2009, 11:08:58 AM »
Yes it can. How did you detect the first antibody? DAB, fluor? Take care in removing any coverslip and staining, as tissue may wash off the slide.
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Paula K. Pierce, HTL(ASCP)HT
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Offline Lee

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Re: Immunostaining
« Reply #2 on: November 11, 2009, 11:17:54 AM »
It was DAB.  Coverslip hadn't even completely dried so removal was easy.  I tried it and it seemed to work.  Thanks!
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Re: Immunostaining
« Reply #2 on: November 11, 2009, 11:17:54 AM »
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