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Author Topic: NG2 and/or PDGFalpha receptor  (Read 2445 times)

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Offline alison jennings

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NG2 and/or PDGFalpha receptor
« on: May 27, 2003, 09:45:41 PM »
Has anyone had success with these antibodies in 4% para or formalin-fixed paraffin sections?
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NG2 and/or PDGFalpha receptor
« on: May 27, 2003, 09:45:41 PM »

Offline richard03

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NG2 and/or PDGFalpha receptor
« Reply #1 on: May 28, 2003, 07:26:23 PM »
Followings are articles using NG2 and PDGF alpha Receptor antibodies on paraffin sections.

1. Expression of Platelet-Derived Growth Factor-A (PDGF-A), PDGF-B, and PDGF Receptor- and -ß during Human Testicular Development and Disease. The Journal of Clinical Endocrinology & Metabolism Vol. 87, No. 5 2310-2319.
http://jcem.endojournals.org/cgi/content/full/87/5/2310

2. Human uveal melanoma expresses NG2 immunoreactivity.  British J. Ophthalmol 2003 May;87(5):629-32
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12714409&dopt=Abstract

Hope this helps.

Richard
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davidtest

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NG2 and/or PDGFalpha receptor
« Reply #2 on: May 29, 2003, 12:00:39 AM »
A polyclonal antibody (PDGFR alpha) from Santa Cruz Biotecnnology (sc-338) works for formalin-fixed, paraffin-embedded tissue sections. A microwave citrate buffer (pH 6.0) pretreatment for 20 minutes is required. Primary antibody at 1:400 dilution for overnight incubation. Skin is a good control tissue.

David
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NG2 and/or PDGFalpha receptor
« Reply #2 on: May 29, 2003, 12:00:39 AM »
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