ceruloplasmin antibody

[Samira]

Hello everybody!

I am looking for a protocol to stain formalin-fixated, paraffin-embedded human liver samples with ceruloplasmin AB.So far, I just heard about cryosections treated with this AB. Because I do not have much material to practise I am very interested in any ideas how to proceed.

Thank you very much !

Samira

[steve]

Where did you purchase the antibody from? Is the datasheet telling you anything about the pretreatment or application or suggested dilution range?

Steve

[Tony]

The following articles may help:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6184594&dopt=Abstract

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2440223&dopt=Abstract

Tony

[Samira]

Hi Steve!

I did not buy an antibody yet because I first wanted to find out more about  this antibody and paraffin sections.


Samira

[steve]

Samira,

Based on Tony's posting, this antibody seemed working well on FFPE tissues. You may want to find original papers for a quick reference.

I have the following suggestions regarding the testing of the antibody:

1) Try three pretreatments: Citrate buffer pH6.0, Tris-EDTA pH9.0 and Trypsin digestion.

2) Use diution recommended by manufacture or set a serial dilutions of primary antibody (i.e. 1:100, 1:500, 1:1000).

3) You should have pretty good chance to get this antibody work.

Good luck,

Steve