Hello-
I am using the Ki-67 monoclonal antibody (clone Ki-s5) on human post-mortem tissue to identify any regions of cell proliferation on diseased human cortical tissue. I used esophagus tissue as a positive control which gave me nice granular staining. I used human control cortical tissue as a negative control and I saw very little staining (although the vessels were highly fluorescent, more so than normal maybe). In my patient tissue, however, I did not see the nice 'granular' staining that I saw with the esophageal tissue, instead I saw regions which contained what looked like staining of 'spider-like' or stellate appearing cells with multiple projections. I've done the stain several times with multiple dilutions (I am using sodium citrate HIER, alexafluor488 anti-mouse secondary.... staining technique works well with other antibodies) but am still seeing these spider-like cells.
Does anyone know of any reports of non-nuclear ki-67 staining in diseased tissue? Is this just an artifact? I do not know what to conclude from these results.
Any input would be greatly appreciated.
Thanks!!
-Nicole
