Suggestions for a strong antibody for neuronal marker for mouse brain tissue?

[bbrim]

Hi,

I am looking for a highly recommended, strong polyclonal antibody against a neuronal  marker to label fresh frozen mouse brain tissue sections (20 microns thick). Because I work in fresh frozen tissue and am doing fluorescent labeling, it needs to be powerful. I prefer a neuronal marker that labels all neurons types as I am trying to differentiate between whether neurons or glia took up a vector expressing my protein of interest. I need it to be  an antibody that is strong and good because funds and time are both limited for any potential troubleshooting. I have already tried an antibody against neuron specific beta tubulin III from abcam, which did not clearly label neurons in tissue.

Thank you so much for your help!

b

[MOzlu]

You can try NeuN (milipore-anti mouse) for neuron detection and S100 beta (abcam-anti rabbit) for glia. I have done a staining with both on 20micron sections recently they work pretty good. I have used 1:1000 for NeuN and 1:2000 for S100beta.
Hope this gives an idea!