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Author Topic: Sucrose Sink  (Read 289 times)

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Offline KCalvin

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Sucrose Sink
« on: February 03, 2012, 10:36:16 PM »
I am currently sectioning brain tissue with the cryostat and I wanted to know if there are adverse effects in leaving the brain tissue in the 30% sucrose sink too long after it has initially sunk?
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Sucrose Sink
« on: February 03, 2012, 10:36:16 PM »

Offline disco volante

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Re: Sucrose Sink
« Reply #1 on: February 06, 2012, 06:36:44 PM »
None that I've seen.  I've had fixed, sucrose cryoprotected brains floating around in the fridge for months and months and they still show good signal in downstream IHC.
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Re: Sucrose Sink
« Reply #1 on: February 06, 2012, 06:36:44 PM »
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