Author Topic: Problem of air bubbles in the cryosectioning  (Read 4038 times)

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Offline WPhillip

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Problem of air bubbles in the cryosectioning
« on: December 24, 2014, 10:51:55 AM »
Hi there,
I encountered a problem for cryosectioning of mouse brain.
After I cut the brain block in the cryostat, I attached those sections to slides.
Then problems occurs. There are some air bubbles trapped in the sections.
Under microscope, these air bubbles appear to be grey.
Although they donot change the morphology, they burst and very destroy the morphology during the in situ hybridization.
Anybody there has an idea how I can solve the problem?
Thank you very much!
Phillip

Problem of air bubbles in the cryosectioning
« on: December 24, 2014, 10:51:55 AM »

Offline gula

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Re: Problem of air bubbles in the cryosectioning
« Reply #1 on: January 01, 2015, 08:58:23 AM »
When you attach the section to the glass-slide, do you warm/melt the section? Or: Do you use cold or warm glass-slides?
I have never seen trapped air-bubbles, when the section melts onto the glass. (My sections are 5-10m)
gula

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« Reply #3 on: July 06, 2020, 03:31:05 AM »