Author Topic: Fixation: Neutral Buffered Formalin vs 4% Paraformaldehyde?  (Read 182 times)

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Offline KMoorwood

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In the past I have used neutral buffered formalin to fix mouse mammary glands prior to IHC and/or IF. Many examples in the literature also specify NBF. However, our lab routinely uses 4% paraformaldehyde for other organs. Does anyone know why I should choose one over the other? Is there something particular about mouse mammary glands that favours NBF?