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Author Topic: NeuN antibody neuronal staining  (Read 5155 times)

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Offline Dierk

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NeuN antibody neuronal staining
« on: February 25, 2005, 09:55:14 AM »
Hi,

I'm planning to use NeuN antibody from mouse to stain rat brain tissue. I want to demonstrate neuronal degeneration of brain after excitotoxic lesions (ibotenic lesions, 6-OHDA etc). Lesions are typically 3 week old.

1. question: is NeuN a better choice than Nissl-Staining? Is it more selective?

2. question: are there special things to take care of with this stain, like fixation etc (I'm planning to do free-floating cryosections on PFA-perfused and shortly postfixated brains)

3. is there a better (= more specific) stain available which you would recommend

Thank you for your input,

dierk
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NeuN antibody neuronal staining
« on: February 25, 2005, 09:55:14 AM »

Offline lldewe

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Fluoro Jade
« Reply #1 on: February 25, 2005, 02:52:58 PM »
Have you tried Fluoro Jade??

Lorie
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Offline Dierk

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NeuN antibody neuronal staining
« Reply #2 on: February 26, 2005, 09:08:07 AM »
Fluoro Jade:

no I have not yet tried this myself. Some coworkers tried it out two years ago, and hat lots of problems with it - but this was before my time, so I do not know what went wrong with it.

Do you use Fluoro Jade on a regular basis?

Any hints would be welcome.

Thx,
Dierk
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Offline lldewe

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Fluoro Jade
« Reply #3 on: March 02, 2005, 07:38:25 PM »
The protocol is so easy it's hard to destroy.  It's published:

http://www.liebertonline.com/doi/pdf/10.1089/neu.2005.22.266;jsessionid=odNP0d66HLf9

If you have any questions I can email you a protocol...

Cheers,
Lorie
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Fluoro Jade
« Reply #3 on: March 02, 2005, 07:38:25 PM »
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