F4/80 staining pattern in mouse spleen?

[DocBob]

I'm testing Serotec's F4/80 mAb on mouse spleen, and see good staining everywhere except the B-cell follicles. Is this the correct pattern? Is this staining pattern published or online anywhere?

Thanks,
DocBob

[richard03]

Check these images for your reference:

http://www.biomedcentral.com/1472-6890/1/3/figure/F1?highres=y

http://www.acris-antibodies.com/focuson/focuson-new-45.html

[broiler25]

Try CD68.  In my opinion it is a far superior marker of macs in mouse tissue.

[DocBob]

Try CD68.  In my opinion it is a far superior marker of macs in mouse tissue.

Is it also on neutrophils, like in humans?

DocBob

[broiler25]

I use clone FA-11-  it is also known as macrosialin, and although referred to as CD68, it does seem to be expressed differently than human CD68.

according to Serotec's spec sheet, Macrosialin 'is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells.'  

also, the following papers I found useful;

Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low densitylipoprotein MysoreP. Ramprasad et al Proc Natl Acad Sci U S A. 1996 December 10; 93(25): 14833–14838.


or


Macrosialin, a Mouse Macrophage-restricted Glycoprotein, Is a Member of the lamp/lgp Family  Holness et al  The Journal of Biological Chemistry Vol 268; 13 9961-9666 1993

'studies reveal a difference between the expression of (human) CD68 and macrosialin; FA/11 staining has only been detected in macrophages, Langerhans cell, and isolated dendritic cells, whereas CD68 is expressed at very low levels in all cell types, but is abundant in macrophages and many tumor cell lines.  / macrosialin may have a role only in macrophages, whereas CD68 may play a role in endocytosis or lysosomal traffic'


I originally used F4/80, but found that I was unable to detect macrophages in the lung that were cearly, morphologically, macrophages.   Since lung is very important to my work, I switched to CD68 and have found it to be a great antibody to work with.