Beta-galactosidase mononclonal

[bn]

Does anyone know of a monoclonal antibody to Beta-galactosidase that works for formaldehyde fixed, paraffin embedded tissue? I want to detect beta-gal in embryonic mouse sections and although I have a good rabbit polyclonal to beta-gal, I need a mouse monoclonal so I can do double labelling with other rabbit antibodies. Alternatively, does anyone have a protocol for double immunofluorescence with two rabbit primary antibodies?
Many thanks in advance.

[richard03]

If you use mouse monoclonal on mouse tissue, it will produce heavy background, especially for embryonic tissues.

I am confused. You are looking for monoclonal beta-gal and double label with rabbit b-gal?

If you use two primary from same species, you should do sequential method, meaning one after another.

Here is a protocol for beta-gal staining

http://www.ihcworld.com/_protocols/antibody_protocols/b-gal_abcam.htm

Richard