Methods and Techniques Discussion => Antigen Retrieval => Topic started by: daiyan1828 on September 22, 2005, 09:08:21 PM

Title: For help! antigen retrieval protocols
Post by: daiyan1828 on September 22, 2005, 09:08:21 PM
Please tell me the protocols on some antigen retrieval solutions ,such as  EDTA-Tris,Tris etc. when using pressure cooker ,how long you keep it?whether the low or high  temperature affects the antigen impression?

daiyan1828@yahoo.com.cn
Title: For help! antigen retrieval protocols
Post by: richard03 on September 23, 2005, 09:16:26 AM
Use the following page for all antigen retrieval protocols:

http://www.ihcworld.com/epitope%20retrieval.htm

Higher temp usually gives stronger staining intensity.

Richard