Methods and Techniques Discussion => Image Analysis => Topic started by: juxianghong on October 25, 2005, 11:34:28 AM

Title: A higher positive rate in apoptosis testing by TUNEL
Post by: juxianghong on October 25, 2005, 11:34:28 AM
Hello:
      I am conducting a research about the endometrium cells apoptosis model during blastocyst implantation in yaks. And the method of apoptosis testing in present study is TUNEL kit(Roche. Germany). The tissue was fixed  by neul-paraformaldehyde and embeded by paraffin. and the antigen retrieval was performed by citrate buffer(PH6.0). But depend on the results of DAB staining, the positive cell in some area attained 40-60%. i beg some suggests to resolve the problems?
Title: A higher positive rate in apoptosis testing by TUNEL
Post by: richard03 on October 25, 2005, 10:36:02 PM
Diluting the TUNEL mixture more may help.