Antibody-Based Technologies => Antibodies Review => Antibody R => Topic started by: quesiluv on December 03, 2005, 01:38:41 PM

Title: RhoA for IH
Post by: quesiluv on December 03, 2005, 01:38:41 PM
Can anyone recommend an antibody for RhoA for use in neuronal cultures?
Title: Re: RhoA for IH
Post by: CincyGrad on May 06, 2013, 10:46:09 AM

I have used the cell signaling RhoA antibody for IHC on paraffin sections and developed it with DAB - it works well and I validated it using knockout tissues as a negative control. The cell signaling website does not list IHC-P as an approved application, but it worked for me! Used the standard 1:200 dilution and sodium citrate for antigen retrieval.