General Research Topics => Molecular Biology => Topic started by: aygroig on January 16, 2006, 03:34:48 AM

Title: florescence vs radioactivity
Post by: aygroig on January 16, 2006, 03:34:48 AM
Hi, i 'd like to know if is possible to use  fluorescence methods to substitute the use of radioactivity in the dna, rna analisys.

thank to all
Title: florescence vs radioactivity
Post by: richard03 on January 17, 2006, 08:44:55 PM
What specific method you are using for DNA or RNA analysis? ISH? If this is the case, I am sure you can use fluorescence methods (FISH) to substitute the use of radioactivity.

Richard
Title: florescence vs radioactivity
Post by: ImmunoNYC on January 18, 2006, 12:06:19 AM
Indeed this is true although expect a decrease in sensitivity which is mostly acceptable especially when probe is good.[/color]

Quote from: "richard03"
What specific method you are using for DNA or RNA analysis? ISH? If this is the case, I am sure you can use fluorescence methods (FISH) to substitute the use of radioactivity.

Richard