Methods and Techniques Discussion => Antigen Retrieval => Topic started by: Evan on March 02, 2006, 11:38:19 AM

Title: 8-ohdg Question
Post by: Evan on March 02, 2006, 11:38:19 AM
So I have been trying to work out a decent stain for 8-ohdg in an oxidative stress model for murine retina, and I was wondering which retrieval method is more appropriate for such a thing - citrate buffer, or proteinase k? The manufacterer recommends proteinase, but the protocol listed here mentions only citrate buffer.

I would think that since 8-ohdg is a nucleic acid stain, one would need to use proteinase k, but any feedback would be appreciated.
Title: 8-ohdg Question
Post by: richard03 on March 02, 2006, 12:02:42 PM
I have used the protocol here and it works well on citrate buffer AR.

Here are some pics using this protocol.

http://ihcworld.com/imagegallery/thumbnails.php?album=search&type=full&search=8-ohdg

Pk may work as some abs work for multiple ARs, but I never tried.

Richard